Figure 6.

Expression of integrins in choroidal melanocytes in culture. The expression of α2, α3, α4, α5, αVβ3, α5β1, β1, β2, β3, β4, β5, and β8 integrins was determined using flow cytometry, as described in Section 2. The shaded areas show staining in the absence of primary antibody (secondary control), and the unshaded areas show staining with primary antibody. The difference between the geometric means of primary antibody-stained cells and secondary antibody control-stained cells can be found in the upper right corner of each graph. These experiments were repeated with two isolations of ChMC with similar results. Please note that α and β integrins were expressed at significant levels with the exception of β3 and αvβ3 integrins.