Figure 10.

Expression of Stem cell factor (Scf) and its receptor (c-Kit) in ChMC. Total RNA was isolated from actively growing ChMC, as well as ChEC, ChPC, RPE, Microglia, REC (retinal EC), and BMMC (bone-marrow-derived mast cells). (A) The expression of Scf and (B) c-Kit was determined by RT-qPCR analysis using specific primers (Table 1). Please note the significant expression of Scf and lower expression of c-Kit in ChMC compared to the other cell types examined. This experiment was repeated with two different isolations of these cells. We also similarly examined the expression of Cfh (complement factor h; (C)) and Htra1 (high-temperature requirement a1; (D) in these cells. Please note significant expression of Cfh and lower expression of Htra1 in ChMC compared to other cell types examined here.