Figure 5.

Immunodetection of dehydrin proteins in leaves (a) and roots (b) of Col-0, ctr1-1 and ein3eil1 plants grown for 21 days on ¹/₂ MS medium −/+ 75 mM sorbitol. Representative images of membranes probed with primary antibodies against the K-, Y-, and S-segments, and Ponceau S staining of total protein loads are shown. The intensities of K- and Y-segment immunoreactive bands in leaves (aligned with the 15, 20, 30, 35, 40, and 45 kDa positions of the protein marker), and K-signal in root samples (at the 60 kDa position), are expressed as values relative to the respective control (quantified with ImageJ 1.52 r).