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. 2025 Apr 27;14(9):642. doi: 10.3390/cells14090642

Figure 2.

Figure 2

Time course for PRLR activation of JAK2/STAT5 pathway in the mediobasal hypothalamus (MBH) of ovariectomized female rats injected with oPRL (100 µg/rat, i.v.). MBH lysates were used for western blot analysis. (A) Top panel: Representative western blot of pJAK2 and JAK2 protein signals. Bottom panel: pJAK2 band density signal normalized to JAK2 band density signal and expressed as percent control. Each value is a mean ± SE of determinations from 6 rats. (B) Top panel: Representative western blot of JAK2 and β-tubulin protein signals. Bottom panel: Total JAK2 protein band density signal normalized to β-tubulin band density signal and expressed as percent control. Each value is a mean ± SE of determinations from 6 rats. (C) Top panel: Representative western blot of pSTAT5 and STAT5 protein signals. Bottom panel: pSTAT5 band density signal normalized to STAT5 band density signal and expressed as percent control. Each value is a mean ± SE of determinations from 8 rats. (D) Top panel: Representative western blot of STAT5 and β-tubulin. Bottom panel: Total STAT5 protein band density signal normalized to β-tubulin band density signal and expressed as percent control. Each value is a mean ± SE of determinations from 8 rats. (A–D) Stippled bars are samples from rats infused with vehicle and black bars are samples from oPRL-treated rats, as indicated in Figure 1A. (E) A representative western blot of MBH lysates was first immunoprecipitated with selective STAT5A (Top panel) or STAT5B (Bottom panel) antibodies and then blotted with pSTAT5 and pan-STAT5 antibodies. MBH tissues were collected at 45 min after oPRL or vehicle injection. (F) Representative western blots of MBH input lysates from above are blotted with the indicated antibodies. * Significantly different (p < 0.05) from the control group.