Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1965 Sep;96(3):626–633. doi: 10.1042/bj0960626

High-efficiency liquid-scintillation counting of 14C-labelled material in aqueous solution and determination of specific activity of labelled proteins

T C Hall 1, E C Cocking 1
PMCID: PMC1207196  PMID: 5862403

Abstract

1. Inexpensive scintillation mixtures are described which enable the detection of as little as 40μμc of 14C in aqueous solution with an efficiency of counting of over 80%. 2. A rapid method for the counting of alkaline, acidic and neutral aqueous solutions of up to 1ml. volume is described. Ethanol or 2-ethoxyethanol is used as blending agent. 3. The scintillation counting of alkaline solutions is applied to the accurate determination of the specific activity of 14C-labelled proteins from plant tissues. 4. Attention has been paid to the importance of a standardized washing procedure for the removal of all traces of radioactive material from glassware.

Full text

PDF
626

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brown W. O. Liquid-scintillation counting of C-labelled animal tissues at high efficiency. Biochem J. 1961 Mar;78(3):571–578. doi: 10.1042/bj0780571. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. EGGSTEIN M., KREUTZ F. H. Vergleichende Untersuchungen zur quantitativen Eiweissbestimmung im Liquor und eiweissarmen Lösungen. Klin Wochenschr. 1955 Oct 1;33(37-38):879–884. doi: 10.1007/BF01473099. [DOI] [PubMed] [Google Scholar]
  3. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  4. ROBINSON C. L., NOVELLI G. D. Binding sites as a limiting factor in amino acid incorporation. Arch Biochem Biophys. 1962 Mar;96:459–464. doi: 10.1016/0003-9861(62)90320-x. [DOI] [PubMed] [Google Scholar]
  5. SIEKEVITZ P. Uptake of radioactive alanine in vitro into the proteins of rat liver fractions. J Biol Chem. 1952 Apr;195(2):549–565. [PubMed] [Google Scholar]
  6. STITCH S. R. Liquid scintillation-counting for [C-14]steroids. Biochem J. 1959 Oct;73:287–292. doi: 10.1042/bj0730287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Smit J. A., Stocken L. A. The effects of x-irradiation on the incorporation of amino acids into proteins of nuclei from rat thymus gland. Biochem J. 1964 Apr;91(1):155–161. doi: 10.1042/bj0910155. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES