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. 2025 May 13;8:745. doi: 10.1038/s42003-025-08162-0

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© The Author(s) 2025

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 1 — a Step 1 in this hiPSC-CM enrichment process is to acquire a phase contrast image of a well of cardiac differentiation with AI detection of CM colonies (red). b Step 2 in this easy-to-use process is replating enriched hiPSC-CMs. c Phase contrast and fluorescent imaging of GFP+ CMs enrichment in 2D monolayers. Successful AI recognition of CMs in phase contrast images was validated using time-lapse imaging of monolayers pre-AI laser processing and post-AI laser processing. The overall GFP confluence did decrease significantly in six independent wells processed using CM-AI-laser ablation of the non-myocyte (GFP-) cell population (Pre GFP confluence = 11.23 ± 3.2%; Post GFP confluence = 8.88 ± 2.4%; n = 6 paired t-test, **P = 0.009). The green Mask was used for quantification of the GFP confluence area before and after CM-AI laser processing. d Validation of CM enrichment in replated monolayers. Following CM-AI laser processing and CM enrichment, CMs were replated on CELLvo™ MatrixPlus human ECM-coated plates for analysis. CM-AI laser-processed wells reached purity (>85%) levels significantly greater than unprocessed wells. (n = 4 monolayers per group; unpaired t-test; ****P < 0.0001).