Fig. 4. Precision and safety of CM-AI laser ablation.

a–d hiPSCs (19-9-11 hiPSC line) were plated as a monolayer on Kataoka Plates and differentiated to CMs. On day 7 hiPSC-CM monolayers were laser cut to make grids (0.4 mm²). a, c phase contrast images show where laser grids were made. b, d Staining with AnnexinV (red apoptosis reagent) shows dead cells are limited to the laser ablated areas. e–h AnnexinV (red) staining following CM-AI and laser processing to purify hiPSC-CMs using two different GFP-tagged cell lines. For each cell line tested, GFP+ CMs are outlined with white dotted line to enhance visualization in (f, h).