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. 2025 May 13;8:745. doi: 10.1038/s42003-025-08162-0

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© The Author(s) 2025

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 6 — a Post-thaw viability was similar between two hiPSC lines tested (TTN-GFP hiPSC-CM = 74.3 ± 7.4; 19.9.11 hiPSC-CM = 61.7 ± 2.9%, n = 3 separate batches per group). b Time-lapse imaging was used to quantify hiPSC-CM 2D monolayer formation kinetics following thaw, data is mean ± standard deviation at each time point. c Phase contrast and GFP images for each cell line tested in panel b on day 3.5 post thaw. d, e Representative calcium transient recordings from each hiPSC line at baseline and with isoproterenol (ISO, 200 nM) treatment. f Each cell line hiPSC-CMs responded to ISO with increased beat rate (TTN-GFP hiPSC-CM baseline = 68.9 ± 8.1; +ISO = 93.3 ± 10.3 bpm, n = 6) (19.9.11 hiPSC-CM baseline = 100.3 ± 15.6; +ISO = 130.0 ± 20.6bpm, n = 6). Paired t-test, ***P = 0.0001. g Each cell line hiPSC-CMs responded to 200 nM ISO with increased calcium wave propagation velocity (TTN-GFP hiPSC-CM baseline = 33.7 ± 5.9; +ISO = 46.9 ± 4.6 cm/s, n = 6) (19.9.11 hiPSC-CM baseline = 30.1 ± 3.4; +ISO = 37.2 ± 6.3 cm/s, n = 6). Paired t-test, ***P = 0.0003; **P = 0.04.