| Thermal cycling protocol CFX96 touch | |||
|---|---|---|---|
| Denaturation cDNA | 95°C | 30 seconds | |
| Denaturation cDNA | 95°C | 5 Seconds | x 40 Cycles |
| Annealing/Extension | 60°C | 30 Seconds | |
| Melt Curve | 65–95°C | 0.5°C increment/5 s | |
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