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. 2025 May 14;20(5):e0312791. doi: 10.1371/journal.pone.0312791

Fig 7. Overexpressing RP11-502I4.3 inhibited the trend of angiogenesis in human retinal microvascular endothelial cells (HRMECs).

Fig 7

(A) Cell Counting Kit-8 assay was performed to determine the cell viability of the control group, high glucose (HG) group, HG + overexpression (OE)-normal control (NC) group, and HG + OE-RP11-502I4.3 group. The experiment was repeated three times in parallel. * p < 0.05. (B) The migrated cells were fixed and stained after the transwell migration assay. (C) The number of migrated cells in each group. The experiment was repeated three times in parallel. * p < 0.05. (D) The images of tube-like structures were observed under an inverted microscope after 24 hours of incubation. (E) The number of tube-forming nodes in each group. (F) The number of tube-forming branches in each group. The above experiments were repeated three times in parallel. * p < 0.05. (G) The expression of vascular endothelial growth factor (VEGF) in human retinal microvascular endothelial cells (HRMECs) after overexpressing the RP11-502I4.3 was analyzed by quantitative real-time polymerase chain reaction. The experiment was repeated three times in parallel. * p < 0.05.