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Identification of novel protein interactions by protein coprecipitation. (A) Pull-down experiment with a control (GST) or target (GST-Borg3) protein using 35S-labeled NIH 3T3 cell lysate. (B) Large-scale affinity purification of GST-Borg3 from the NIH 3T3 lysate. Individual proteins were microsequenced by mixed-peptide sequencing and identified by database searching with the FASTF algorithm (101).
