Figure 3.

The AAV-GAD67 construct used in this study transfected neuronal and non-neuronal cells. (A) Diagram of the AAV construct used. (B) Representative confocal images illustrating AAV-GAD67 expression (EGFP, yellow) in combination with NeuN (cyan) and Pax2 (magenta). Enlarged views of the region marked by the white box in the main image are shown in the middle and lower panel, with arrows highlighting co-localized cells. (C) Distribution of viral expression across individual laminae, represented in mm² (left) and as a percentage of the total area of each defined region (right). (D) Fraction of AAV⁺ cells within each lamina co-expressing NeuN or Pax2. (E) Representative image of AAV-GAD67 transfected cells (yellow) without NeuN (cyan) co-labeling (arrow). (F,G) Transduction efficiency, presented as the percentage of EGFP⁺ cells per mm² relative to the total NeuN⁺ (F) or Pax2⁺ (G) populations. n = 7 to 8 slices from 3 animals. Data show mean ± SEM.