TABLE 4.
Primary active transporters: ABC transportersa
| Family and transporter | TC no. | ORF | No. of TMSs | Putative substrate | Occurrence | Commentsb | E value | PRSS P value | Xylella annotation | COG |
|---|---|---|---|---|---|---|---|---|---|---|
| Uptake systems | ||||||||||
| CUT1 | 3.A.1.1.3 | XF1067 | 0 | Sugar | B | Glycerol-phosphate porter; cytoplasm (ATP binding) | 4e-94 | 5.2223e-64 | Sugar ABC transporter ATP-binding protein | COG1130 (175 proteins), ABC-type sugar/spermidine/putrescine/iron/thiamine transport systems, ATPase component |
| CUT1 | 3.A.1.1.4 | XF2446 | 5 | Sugar | B | Lactose porter; membrane | 1e-42 | 2.5711e-23 | ABC transporter sugar permease | COG0395 (111 proteins), sugar permeases |
| CUT1 | 3.A.1.1.4 | XF2447 | 4 | Sugar | B | Lactose porter; membrane | 6e-52 | 5.0915e-32 | ABC transporter sugar permease | COG1175 (110 proteins), ABC-type sugar transport systems, permease components |
| CUT1 | 3.A.1.1.7 | XF2448 | 0 | Sugar | B | Maltose/trehalose porter; receptor | 5e-25 | 5.5258e-22 | ABC transporter sugar binding protein | COG1653 (83 proteins), sugar-binding periplasmic proteins/domains |
| PAAT | 3.A.1.3.1 | XF0875 | 0 | Polar a.a. | Histidine/arginine/lysine/ornithine porter; cytoplasm (ATP binding); lacks R | 5e-45 | 1.7196e-35 | ABC transporter ATP binding protein | COG1135 (24 proteins), uncharacterized ABC-type transport system ATPase component | |
| PAAT | 3.A.1.3.2 | XF2695 | 0 | Polar a.a. | Glutamine porter; ATP binding ABC domain | 1e-36 | 1.3515e-28 | ABC transporter ATP binding protein | COG2884 (18 proteins), predicted ATPase involved in cell division | |
| PAAT | 3.A.1.3.4 | XF0874 | 4 | Polar a.a. | Glutamate/aspartate porter; membrane; lacks R | 4e-07 | 2.7183e-08 | ABC transporter permease protein | COG2011 (22 proteins), permease component of an uncharacterized ABC transporter | |
| PAAT | 3.A.1.3.9 | XF0420 | 3 | Glutamate? | Membrane | Toluene tolerance protein | COG0767 (32 proteins), ABC-type toluene export system, permease component | |||
| PAAT | 3.A.1.3.9 | XF0418 | 0 | Glutamate? | Receptor | Toluene tolerance protein | COG2854 (12 proteins), uncharacterized periplasmic protein | |||
| PAAT | 3.A.1.3.9 | XF0419 | 0 | Glutamate? | Receptor | Toluene tolerance protein | COG1463 (53 proteins), permease component of an ABC transporter | |||
| PAAT | 3.A.1.3.9 | XF0421 | 0 | Glutamate | Similar to glutamate porter GluA of Corynebacterium; cytoplasm (ATP binding) | 2e-29 | 2.9871e-27 | Toluene tolerance protein | COG1127 (24 proteins), uncharacterized ABC-type transport system, ATPase component | |
| HAAT | 3.A.1.4.1 | XF1409 | 0 | Leu/Ile/Val | Leu/Ile/Val porter (hydrophobic amino acid uptake); cytoplasm (ATP binding), lacks M, R | 2e-31 | 5.8503e-26 | ABC transporter ATP-binding protein | COG1137 (24 proteins), ABC-type (unclassified) transport systemATPase component | |
| PepT | 3.A.1.5.3 | XF1475 | 0 | Nickel | Nickel porter; cytoplasm (ATP binding), lacks M, R | 1e-14 | 2.0481e-15 | ABC transporter ATP-binding protein | COG0396 (28 proteins), iron-regulated ABC transporter ATPase subunit SufC | |
| SulT | 3.A.1.6.1 | XF1345 | 6 | Sulfate | B | Sulfate/thiosulfate porter membrane | 8e-75 | 8.6038e-42 | ABC transporter sulfate permease | COG0555 (55 proteins), ABC-type sulfate/molybdate transport systems, permease components |
| SulT | 3.A.1.6.1 | XF1346 | 5 | Sulfate | B | Sulfate/thiosulfate porter membrane | 2e-71 | 5.8465e-39 | ABC transporter sulfate permease | COG0555 (55 proteins), ABC-type sulfate/molybdate transport systems, permease components |
| SulT | 3.A.1.6.1 | XF1344 | 0 | Sulfate | B | Sulfate/thiosulfate porter receptor | e-119 | 7.8369e-94 | ABC transporter sulfate-binding protein | COG1613 (17 proteins), ABC-type sulfate transport system, periplasmic component |
| SulT | 3.A.1.6.1 | XF1347 | 0 | Sulfate | B | Sulfate/thiosulfate porter; cytoplasm (ATP binding) | 8e-80 | 2.6395e-56 | sulfate ABC transporter ATP-binding protein | COG1118 (29 proteins), ABC-type sulfate/molybdate transport systems, ATPase component |
| PhoT | 3.A.1.7.1 | XF2142 | 5 | Phosphate | B | Phosphate porter; membrane | 5e-90 | 1.3785e-40 | ABC transporter phosphate permease | COG0573 (34 proteins), ABC-type phosphate transport system, permease component |
| PhoT | 3.A.1.7.1 | XF2143 | 5 | Phosphate | B | Phosphate porter; membrane | 9e-86 | 3.1053e-48 | ABC transporter phosphate permease | COG0581 (34 proteins), ABC-type phosphate transport system, permease component |
| PhoT | 3.A.1.7.1 | XF2144 | 1 | Phosphate | B | Phosphate porter; cytoplasm (ATP binding) | 1e-97 | 4.0756e-75 | Phosphate ABC transporter ATP-binding protein | COG1117 (38 proteins) ABC-type phosphate transport system, ATPase component |
| PhoT | 3.A.1.7.1 | XF2141 | 0 | Phosphate | B | Phosphate porter; receptor | 4e-95 | 2.4942e-50 | ABC transporter phosphate-binding protein | COG0226 (46 proteins) ABC-type phosphate transport system, periplasmic component |
| VB12T | 3.A.1.13.1 | XF1604 | 0 | Vitamin B12 | B | Vitamin B12 porter; receptor; lacks C, M | 2e-35 | 1.0273e-33 | ABC transporter vitamin B12 uptake permease | COG0386 (25 proteins) Glutathione peroxidase |
| NitT | 3.A.1.16.3 | XF0411 | 8 | Bicarbonate | B | Bicarbonate porter; membrane; lacks R | 2e-15 | 7.395e-11 | ABC transporter nitrate permease | COG0600 (50 proteins) ABC-type nitrate/sulfonate/taurine/bicarbonate transport systems, permease components |
| NitT | 3.A.1.16.3 | XF0412 | 0 | Bicarbonate | B | Bicarbonate porter; cytoplasm (ATP-binding); lacks R | 2e-44 | 4.6747e-33 | Nitrate ABC transporter ATP-binding protein | [2 domains] (1) XF0412_1: COG1116 (49 proteins) ABC-type nitrate/sulfonate/taurine/bicarbonate transport systems, ATPase components; (2) XF0412_2: NO COG |
| Export systems | ||||||||||
| LOSE | 3.A.1.102.1 | XF1602 | 1 | Lipooligosaccharide | B | Lipooligosaccharide exporter (nodulation protein); cytoplasm (ATP binding), lacks M | 1e-18 | 1.509e-17 | ABC transporter ATP-binding protein | COG1131 (228 proteins) ABC-type multidrug transport system, ATPase component |
| LOSE | 3.A.1.102.1 | XF1302 | 0 | Lipooligosaccharide | B | Lipooligosaccharide exporter (nodulation protein); cytoplasm (ATP binding), lacks M | 2e-28 | 4.5271e-23 | ABC transporter ATP-binding protein | COG1127 (24 proteins) Uncharacterized ABC-type transport system, ATPase component |
| LPSE | 3.A.1.103.1 | XF2567 | 5 | Lipopolysaccharide | B | Lipopolysaccharide exporter; membrane | 1e-13 | 1.844e-09 | ABC transporter permease protein | COG1682 (27 proteins) Membrane permeases involved in cell wall biosynthesis |
| LPSE | 3.A.1.103.1 | XF2568 | 0 | Lipopolysaccharide | B | Lipopolysaccharide exporter; cytoplasm (ATP binding) | 4e-45 | 4.8821e-39 | ABC transporter ATP-binding protein | COG1134 (25 proteins) ABC-type polysaccharide/polyol phosphate transport system, ATPase component |
| DrugE1 | 3.A.1.105.1 | XF1222 | 5 | Drug | B | Daunorubicin; doxorubicin (drug resistance) exporter; membrane | 5e-06 | 2.3754e-07 | Conserved hypothetical protein | COG0842 (129 proteins) ABC-type multidrug transport system, permease component |
| DrugE1 | 3.A.1.105.1 | XF1223 | 0 | Drug | B | Daunorubicin; doxorubicin (drug resistance) exporter; cytoplasm (ATP binding) | 2e-36 | 1.189e-27 | ABC transporter ATP-binding protein | COG1131 (228 proteins), ABC-type multidrug transport system, ATPase component |
| LipidE | 3.A.1.106.1 | XF1081 | 6 | Lipid | B | Putative lipid A exporter (flippase); cytoplasm (ATP binding), plus M together | e-131 | 5.5729e-93 | ABC transporter ATP-binding protein | COG1132 (204 proteins), ABC-type multidrug/protein/lipid transport system ATPase component |
| LipidE | 3.A.1.106.1 | XF2582 | 3 | Lipid | B | Putative lipid A exporter (flippase); cytoplasm (ATP binding), plus M together | 2e-84 | 1.3328e-59 | ABC transporter ATP-binding protein | COG1132 (204 proteins), ABC-type multidrug/protein/lipid transport system, ATPase component |
| HemeE | 3.A.1.107.1 | XF2456 | 7 | Heme | B | Putative heme exporter; membrane | 2e-41 | 1.0691e-19 | Heme ABC transporter membrane protein | COG2386 (11 proteins), ABC-type transport system involved in cytochrome c biogenesis, permease component |
| HemeE | 3.A.1.107.1 | XF2457 | 6 | Heme | B | Putative heme exporter; membrane | 2e-49 | 3.8318e-30 | Heme ABC transporter membrane protein | COG0755 (36 proteins), ABC-type transport system involved in cytochrome c biogenesis, permease component |
| HemeE | 3.A.1.107.1 | XF2455 | 0 | Heme | B | Putative heme exporter; cytoplasm (ATP binding) | 2e-20 | 1.718e-21 | Heme ABC transporter ATP-binding protein | COG1131 (228 proteins), ABC-type multidrug transport system, ATPase component |
| Prot1E | 3.A.1.109.1 | XF2397 | 5 | Protein | B | α-Hemolysin exporter; cytoplasm (ATP binding), plus M together | 0.0 | 2.5016e-148 | Toxin secretion ABC transporter ATP-binding protein | COG2274 (19 proteins), ABC-type bacteriocin/lantibiotic exporters, contain an N-terminal double glycine peptidase domain |
| Prot2E | 3.A.1.110.2 | XF1220 | 5 | Protein | B | Colicin V exporter; cytoplasm (ATP binding), plus M together | e-152 | 1.0121e-102 | Colicin V secretion ABC transporter ATP-binding protein | COG2274 (19 proteins), ABC-type bacteriocin/lantibiotic exporters, contain an N-terminal double glycine peptidase domain |
| DevE | 3.A.1.114.1 | XF1077 | 0 | Glycolipid | B | Probable glycolipid exporter (envelope formation); cytoplasm (ATP binding) lacks M | 2e-38 | 1.2501e-31 | ABC transporter ATP-binding protein | COG1136 (121 proteins), ABC-type transport systems, involved in lipoprotein release, ATPase components |
| NatE | 3.A.1.115.1 | XF2328 | 6 | Sodium | B | ABC transporter sodium permease; membrane | 9e-32 | 1.2142e-23 | ABC transporter sodium permease | COG1668 (20 proteins), ABC-type Na+ efflux pump, permease component |
| NatE | 3.A.1.115.1 | XF2329 | 1 | Sodium | B | Sodium ABC transporter ATP-binding protein; cytoplasm (ATP binding) | 1e-59 | 1.2537e-46 | Sodium ABC transporter ATP-binding protein | COG1131 (228 proteins), ABC-type multidrug transport system, ATPase component |
| DrugRA1 | 3.A.1.120.1 | XF2617 | 1 | Drugs | B | Macrolide ATPase; cytoplasm (ATP-binding), lacks M | 3e-59 | 2.2893e-39 | ABC transporter ATP-binding protein | COG0488 (93 proteins), ATPase components of ABC transporters with duplicated ATPase domains |
| DrugRA1 | 3.A.1.120.1 | XF2133 | 0 | Drugs | B | Macrolide ATPase; cytoplasm (ATP binding) | 9e-51 | 8.1429e-35 | ABC transporter ATP-binding protein | COG0488 (93 proteins), ATPase components of ABC transporters with duplicated ATPase domains |
| DrugRA1 | 3.A.1.120.2 | XF0944 | 1 | Drugs | B | Tylosin ATPase; cytoplasm (ATP binding) | 2e-58 | 4.0421e-41 | ABC transporter ATP-binding protein | COG0488 (93 proteins), ATPase components of ABC transporters with duplicated ATPase domains |
a
See Table 2, footnote a.
b
C, cytoplasmic subunit; M, membrane component; R, receptor component.