TABLE 5.

Summary of cellulase adsorption parameters^a

Organism, substrate and temp	Binding capacity			Binding affinity		Reference
	mg/g	μmol/g	μmol/μmolb	Liters/g	Liters/μmol
Components
Cellulomonas fimi (30°C)
CenA BMCC	144	3.1	5.0 × 10−4	41	1.89	212
Cex BMCC	184	3.6	5.8 × 10−4	33	1.71
Thermobifida fusca (50°C)
E3, Avicel	26	0.4	6.5 × 10−5	3.1	0.20	70
E3, BMCC	741	11.4	1.8 × 10−3	1.5	0.10
E4, Avicel	31	0.34	5.5 × 10−5	0.85	0.077
E4, BMCC	875	9.7	1.6 × 10−3	0.49	0.044
E5, Avicel	31	0.67	1.1 × 10−4	4.8	0.22
E5, BMCC	556	12.0	1.9 × 10−3	2.8	0.13
Trichoderma reesei
CBHI, Avicel (4°C)	48	0.74	1.2 × 10−4	0.69	0.044	439
CBHI, Avicel (25°C)	57	1.1	1.8 × 10−4	5.4	0.28	637
CBHI, Avicel (25°C)	15	0.29	4.7 × 10−5			682
CBHI, Avicel (50°C)	25	0.48	7.8 × 10−5	1.7	0.09	70
CBHI/BMCC (50°C)	239	4.6	7.5 × 10−4	5.4	0.28	70
CBHII, Avicel (4°C)	28	0.52	8.4 × 10−5	1.0	0.053	439
CBHII, Avicel (25°C)	11.3	0.24	3.9 × 10−5			682
Trichoderma viride (30°C)
ExoI	6.6	0.11	1.8 × 10−5	5.0	0.3	44
ExoIII	63	1.0	1.6 × 10−4	6.9	0.43
EndoI, Avicel	130	2.5	4.0 × 10−4	0.88	0.04
EndoIII, Avicel	26	0.45	7.3 × 10−5	12	0.68
EndoV, Avicel	110	1.8	2.9 × 10−4	0.89	0.05
EndoVI, Avicel	4.1	0.08	1.3 × 10−5	3.4	0.18
Multicomponent mixtures and complexes
Trichoderma reesei
Avicel (50°C)	92	1.92	3.1 × 10−4	1.04	0.05	641
Avicel (40°C)	56	1.21	2.0 × 10−4	3.21	0.015	508
Cellulose in pretreated woodc (40°C)	81	1.68	2.7 × 10−4	1.82	0.087	506
C. thermocellum
Avicel (60°C)d	17.5	0.0083	1.4 × 10−5	246	517	50
Pretreated wood(60°C)d,e	317	0.15	2.5 × 10−5	344	722

^aValues in bold are as reported; others are calculated.

^bMicromole of cellulase/micromole of β-glucosidic bond calculated using 6,173 μmol of β-glucosidic bonds/g of cellulose.

^cDilute-acid-pretreated wood prepared at 220°C; an average molecular weight of 48,000 is assumed as in reference 641.

^dCalculated quantities based on a specific activity of 2.4 μmol/mg/min and a molecular mass of 2.1 × 10⁶ Da.

^eDilute-acid-pretreated wood prepared at 220°C.