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. 2002 Sep;66(3):460–485. doi: 10.1128/MMBR.66.3.460-485.2002

FIG. 3.

FIG. 3.

(A) Secondary structure of the initiation site of the MS2 RNA coat protein cistron, sequence of the start site of the 200-bp unstructured mRNAs, and sequence of the start site of the unstructured 20-bp mRNA. (B) Ribosome-mRNA complexes with unstructured mRNAs or with the structured MS2 RNA. (A) W2 binds unstructured mRNAs (lanes 1 and 2, 20 and 200 bp of [32P]mRNA; lanes 4 and 5, 20 and 200 bp of [32P]mRNA plus 0.2 μg of W2). (B) Binding of 200 bp of unstructured [32P]mRNA to ribosomes with 0, 0.1, 0.2, and 0.4 μg of W2 (lanes 1 to 4). (C) Ternary initiation complexes, f[35S]Met-tRNA-ribosome-MS2 RNA, require W2. (D) Ternary initiation complexes, fMet[35S]Met-tRNA-ribosome-unstructured mRNA, do not require W2 (lane 1, no mRNA; lane 2, 200 bp of mRNA; lane 3, 200 bp of mRNA plus 0.1 μg of W2; lane 4, 20 bp of mRNA; lane 5, 20 bp of mRNA plus 0.1 μg of W2). See reference 122 for experimental details.