Table 2. Volatile Substances Profile of G. mellonella Egg Extracts (EE) by GC-MSa.
|
peak
area (%) |
||||||||
|---|---|---|---|---|---|---|---|---|
| peak number | RT (min) | C | GP | GN | compound name | MW | formula | |
| EE1 | 1 | 17.835 | 3.8 | 4.8 | 5.1 | tetradecanal | 212 | C14H28O |
| 2 | 18.935 | 2.8 | 1.9 | 2.8 | tridecanoic acid, methyl ester | 228 | C14H28O2 | |
| 3 | 19.250 | 9.7 | 13.0 | 14.7 | hexadecanoic acid, methyl ester | 270 | C17H34O2 | |
| 4 | 19.600 | 1.8 | 10.4 | 9.1 | hexadecanoic acid, ethyl ester | 284 | C18H36O2 | |
| 5 | 19.880 | 6.8 | 7.3 | 7.6 | octadecanal | 268 | C18H36O | |
| 6 | 20.855 | 2.4 | 1.7 | 1.8 | methyl stearate | 298 | C19H38O2 | |
| 7 | 21.155 | 9.3 | 12.0 | 11.8 | octadecanoic acid | 284 | C18H36O2 | |
| 8 | 21.250. | 7.4 | 19.2 | 13.8 | ethyl oleate | 310 | C20H38O2 | |
| 9 | 21.595 | 1.5 | 1.5 | 3.8 | heptacosyl acetate | 438 | C29H58O2 | |
| 10 | 23.150 | 12.0 | 3.4 | 2.4 | nonadecanamide | 297 | C19H39NO | |
| 11 | 24.105 | 8.7 | 9.4 | 7.6 | heneicosane | 269 | C21H44 | |
| 12 | 24.185 | 25.6 | 1.5 | 2.5 | dotriacontane | 450 | C32H66 | |
| 13 | 24.725 | 3.1 | 4.5 | 11.9 | bis(2-ethylhexyl) phthalate | 390 | C24H38O4 | |
| 14 | 26.405 | 5.0 | 9.5 | 5.1 | tetracontane | 562 | C40H82 | |
| EE2 | 1 | 17.840 | 0.7 | 0.9 | 0.6 | tetradecanal | 212 | C14H28O |
| 2 | 18.435 | 0.3 | 0.3 | 0.2 | 1,2-benzenedicarboxylic acid, bis(2-methylpropyl) ester | 278 | C16H22O4 | |
| 3 | 18.935 | 1.5 | 0.9 | 0.7 | hexadecanoic acid, methyl ester | 270 | C17H34O2 | |
| 4 | 19.255 | 4.0 | 5.0 | 8.9 | n-hexadecanoic acid | 256 | C16H32O2 | |
| 5 | 19.600 | 2.9 | 2.2 | 3.4 | hexadecanoic acid, ethyl ester | 284 | C18H36O2 | |
| 6 | 19.895 | 0.7 | 1.0 | 0.8 | octadecanal | 268 | C18H36O | |
| 7 | 20.600 | 0.6 | 0.5 | 1.1 | 9,12-octadecadienoic acid (Z,Z)–, methyl ester | 294 | C19H34O2 | |
| 8 | 20.645 | 1.6 | 0.8 | 1.2 | 9-octadecenoic acid, methyl ester, (E)– | 296 | C19H36O2 | |
| 9 | 20.860 | 1.5 | 0.9 | 0.7 | methyl stearate | 298 | C19H38O2 | |
| 10 | 21.165 | 5.3 | 5.6 | 9.6 | octadecanoic acid | 284 | C18H36O2 | |
| 11 | 21.255 | 10.5 | 6.2 | 9.3 | ethyl oleate | 310 | C20H38O2 | |
| 12 | 21.595 | 2.2 | 1.2 | 0.3 | heptacosyl acetate | 438 | C29H58O2 | |
| 13 | 22.360 | 4.7 | 3.3 | 3.7 | heneicosane | 296 | C21H44 | |
| 14 | 24.105 | 25.7 | 25.7 | 21.3 | pentacosane | 352 | C25H52 | |
| 15 | 26.405 | 25.9 | 29.8 | 25.8 | dotriacontane | 450 | C32H66 | |
| 16 | 29.770 | 11.9 | 15.8 | 12.4 | tetracontane | 562 | C40H82 | |
| EE3 | 1 | 17.835 | 2.5 | 3.0 | 2.8 | tetradecanal | 212 | C14H28O |
| 2 | 18.435 | 1.0 | 1.2 | 1.5 | 1,2-benzenedicarboxylic acid, bis(2-methylpropyl) ester | 278 | C16H22O4 | |
| 3 | 19.255 | 28.8 | 25.7 | 25.9 | n-hexadecanoic acid | 256 | C16H32O2 | |
| 4 | 19.600 | 5.1 | 7.3 | 5.9 | hexadecanoic acid, ethyl ester | 284 | C18H36O2 | |
| 5 | 19.890 | 2.8 | 2.8 | 3.6 | hexadecanal | 240 | C16H32O | |
| 6 | 20.895 | 1.9 | 1.0 | 1.1 | methyl stearate | 298 | C19H38O2 | |
| 7 | 21.150 | 38.1 | 40.6 | 46.0 | octadecanoic acid | 284 | C18H36O2 | |
| 8 | 21.590 | 5.6 | 3.5 | 3.6 | heptacosyl acetate | 438 | C29H58O2 | |
| 9 | 23.550 | 6.7 | - | - | phenol, 2,2′-methylenebis[6-(1,1-dimethylethyl)-4-methyl | 340 | C23H32O2 | |
| 10 | 24.720 | 7.5 | 15.0 | 9.6 | bis(2-ethylhexyl) phthalate | 390 | C24H38O4 | |
a
RT: retention time; MW: molecular weight; -: absence; C: eggs of larvae from the control group; GP: eggs of larvae that received antigens of Gram-positive bacteria; and GN: eggs of larvae that received antigens of Gram-negative bacteria.