FIG. 3.

Supershift assay. The full-length, intermediate, and processed PacC-DNA complexes can be resolved in an EMSA gel. The open (intermediate) form has its regions A and B available for interaction with a purified glutathione S-transferase (GST) fusion protein (here GST::PacC[410-678]) containing interacting region C, whereas in full-length, closed PacC these regions are not available (see the schematic representations). The remarkably stable interaction of open PacC with the large (possibly dimeric) GST fusion protein results in markedly reduced mobility (i.e., supershift) of the corresponding protein-DNA complex. The presence or absence from the reaction mixture of the GST::PacC[410-678] fusion protein is indicated by + and −, respectively.