Figure 3.

Phenotypic activity profile of PB94. (A) Reference benchmark overlay of PB94 (3.3 μM) and vorinostat (3.3 μM); (B) top database search result for PB94 (3.3 μM) is parthenolide (1.2 μM); (C) HeatMAP analysis for PB94. HeatMAP analysis of the 148 biomarker readouts (rows) within the Diversity PLUS Panel by PB94 in comparison to 19 consensus mechanism class profiles (columns); (D) mechanism of action by which PB94 affects neuroinflammatory events was characterized by a focus on IL-10 using mouse microglia BV2 cells. BV2 cells were utilized to assess inflammatory changes induced by lipopolysaccharides (LPS). Cells were treated with LPS (10 ng/mL) alone or in combination with 500 or 1000 nM PB94 for 24 h. The medium was then applied to the MSD-cytokine assay to measure levels of IL-10. mean ± standard error of the mean (SEM); t-test; n = 4 for each group.