Reagents and tools table
| Reagent/Resource | Reference or Source | Identifier or Catalog Number |
|---|---|---|
| Experimental models | ||
| C57BL/6J | Jackson lab | Strain #:000664 |
| B6;129S4-Tet1tm1.1Jae/J | Jackson lab | Strain #:017358 |
| B6.Cg-Speer6-ps1Tg(Alb-cre)21Mgn/J | Jackson lab | Strain #:003574 |
| B6N.FVB-Tmem163Tg(ACTB-cre)2Mrt/CjDswJ | Jackson lab | Strain #:019099 |
| TET1 floxed mice | PMID: 26199412, Dr. Anjana Rao | Not applicable |
| OUMS29 | PMID: 11513080 | Not applicable |
| HEK-293T | ATCC | CRL-3216™ |
| Recombinant DNA | ||
| shRNA-luciferase | Addgene | #78162 |
| shRNA-TET1#23 | Horizon | RHS3979-201792087 |
| shRNA-TER1#26 | Horizon | RHS3979-201797038 |
| pcDNA3.1-Flag | Addgene | #208051 |
| pcDNA3-Tet1 | Addgene | #60938 |
| p1D4-hrGFP II | Addgene | #71317 |
| pIRES-hrGFP II-mTET1 | Addgene | #83569 |
| pIRES-hrGFP II-TET1_CD | Addgene | #83570 |
| pIRES-hrGFP II-TET1 | Addgene | #83568 |
| pLenti-III-HA | Applied Biological Materials Inc. | LV587 |
| pLenti-III-HA-CD36 | Applied Biological Materials Inc. | 155340610295 |
| pGL4.24-Cd36-enhancer2 | Addgene | #138574 |
| pGL4.24-Cd36-enhancer3 | Addgene | #138575 |
| pGL4.24-Cd36-enhancer4 | Addgene | #138576 |
| pGL4.24-Cd36-enhancer5 | Addgene | #138577 |
| pGL4.24-Cd36-enhancer7 | Addgene | #138579 |
| pGL4.11-Cd36-promoter | Addgene | #138572 |
| Antibodies | ||
| TET1 Polyclonal antibody for human (1:1000) | ThermoFisherScientific | PA5-85489 |
| TET1 antibody for mouse (1:1000) | SigmaMillipore | SAB270073 |
| TET2 antibody (1:1000) | Active Motif | 61389 |
| TET3 antibody (1:1000) | Genetex | GTX121453 |
| GAPDH antibody (1:100,000) | ABclonal Technology | A19056 |
| Akt antibody (1:1000) | Cell Signaling Technology® | 4691S |
| pAkt antibody (1:1000) | Cell Signaling Technology® | 4060S |
| AMPK antibody (1:1000) | Cell Signaling Technology® | 2532S |
| pAMPK antibody (1:1000) | Cell Signaling Technology® | 2535S |
| PGC1a antibody (1:1000) | Cell Signaling Technology® | 2178 |
| a-tublin antibody (1:6000) | ABclonal Technology | A6830 |
| CD36 antibody (1:500) | Proteintech | 32181-1-AP |
| LKB1 antibody (1:1000) | Cell Signaling Technology® | 3050S |
| pLKB1 antibody (1:1000) | Cell Signaling Technology® | 3482S |
| 5-methylcytosine antibody (1:1000) | Active Motif | 61479 |
| 5-hydroxylmethylcytosine antibody (1:10,000) | Active Motif | 39769 |
| Oligonucleotides and other sequence-based reagents | ||
| Human TET1-Forward (F) | Integrated DNA Technologies | CAGAACCTAAACCACCCGTG |
| Human TET1-Reverse (R) | Integrated DNA Technologies | TGCTTCGTAGCGCCATTGTAA |
| Human IGFBP2-F | Integrated DNA Technologies | CAGACGCTACGCTGCTATCC |
| Human IGFBP2-R | Integrated DNA Technologies | CCCTCAGAGTGGTCGTCATCA |
| Human CD36-F | Integrated DNA Technologies | GGCTGTGACCGGAACTGTG |
| Human CD36-R | Integrated DNA Technologies | AGGTCTCCAACTGGCATTAGAA |
| Mouse Tet1-F | Integrated DNA Technologies | ACACAGTGGTGCTAATGCAG |
| Mouse Tet1-R | Integrated DNA Technologies | AGCATGAACGGGAGAATCGG |
| Mouse Tet2-F | Integrated DNA Technologies | AGAGAAGACAATCGAGAAGTCGG |
| Mouse Tet2-R | Integrated DNA Technologies | CCTTCCGTACTCCCAAACTCAT |
| Mouse Tet3-F | Integrated DNA Technologies | TGCGATTGTGTCGAACAAATAGT |
| Mouse Tet3-R | Integrated DNA Technologies | TCCATACCGATCCTCCATGAG |
| Mouse Cd36-F | Integrated DNA Technologies | CCA GTC GGA GAC ATG CTT ATT |
| Mouse Cd36-R | Integrated DNA Technologies | GTA CAC AGT GGT GCC TGT T |
| Mouse Igfbp2-F | Integrated DNA Technologies | TGGAACATCTCTACTCCCTGC |
| Mouse Igfbp2-R | Integrated DNA Technologies | CTTCCCGGTATTGGGGTTCA |
| Mouse IGFBP3-F | Integrated DNA Technologies | CCA GGA AAC ATC AGT GAG TCC |
| Mouse IGFBP3-R | Integrated DNA Technologies | GGA TGG AAC TTG GAA TCG GTC A |
| Mouse Enho-F | Integrated DNA Technologies | CTC ATC GCC ATC GTC TGC AAT |
| Mouse Enho-R | Integrated DNA Technologies | GGG ACT GGA TTC CGA GAG AGA |
| Mouse Eda-F | Integrated DNA Technologies | CAA GGG TCA GCA ATT CAA GTC A |
| Mouse Eda-R | Integrated DNA Technologies | CAC CTT AGG GTT CAT AGT GAT GC |
| Mouse HSP90AA1-F | Integrated DNA Technologies | TGT TGC GGT ACT ACA CAT CTG C |
| Mouse HSP90AA1-R | Integrated DNA Technologies | GTC CTT GGT CTC ACC TGT GAT A |
| Mouse SLC27A1-F | Integrated DNA Technologies | TCT GTT CTG ATT CGT GTT CGG |
| Mouse SLC27A1-R | Integrated DNA Technologies | CAG CAT ATA CCA CTA CTG GCG |
| Mouse HNF4-A-F | Integrated DNA Technologies | GTG GCG AGT CCT TAT GAC ACG |
| Mouse HNF4-A-R | Integrated DNA Technologies | GCT GTT GGA TGA ATT GAG GTT GG |
| Mouse PPAR-A-F | Integrated DNA Technologies | TTT CGG CGA ACT ATT CGG CTG |
| Mouse PPAR-A-R | Integrated DNA Technologies | GGC ATT TGT TCC GGT TCT TCT T |
| Mouse CPT1b-F | Integrated DNA Technologies | TCT TCT TCC GAC AAA CCC TGA |
| Mouse CPT1b-R | Integrated DNA Technologies | GAG ACG GAC ACA GAT AGC CC |
| Mouse CPT1a-F | Integrated DNA Technologies | CTA TGC GCT ACT CGC TGA AGG |
| Mouse CPT1a-R | Integrated DNA Technologies | GGC TTT CGA CCC GAG AAG A |
| Mouse CrAT-F | Integrated DNA Technologies | CTC CTG GGC TGG AGT AGA TG |
| Mouse CrAT-R | Integrated DNA Technologies | TTA CAG AAG GGA CTG GAG CG |
| Mouse OCTN2-F | Integrated DNA Technologies | AAG ACC TGC AGG AAG CTG AA |
| Mouse OCTN2-R | Integrated DNA Technologies | TCC TTG TTT TTC GTG GGT GT |
| Mouse CYP4A10-F | Integrated DNA Technologies | TCC AGC AGT TCC CAT CAC CT |
| Mouse CYP4A10-R | Integrated DNA Technologies | TTG CTT CCC CAG AAC CAT CT |
| Mouse CYP4A14-F | Integrated DNA Technologies | TCA GTC TAT TTC TGG TGC TGT TC |
| Mouse CYP4A14-R | Integrated DNA Technologies | GAG CTC CTT GTC CTT CAG ATG GT |
| Mouse EHHADH-F | Integrated DNA Technologies | CGT CTC CTC GGT TGG TGT TC |
| Mouse EHHADH-R | Integrated DNA Technologies | ATT ATC TTC TTT GCA GTA TCT AGC TGC TT |
| Mouse FGF21-F | Integrated DNA Technologies | AAA GCC TCT AGG TTT CTT TGC CA |
| Mouse FGF21-R | Integrated DNA Technologies | CCT CAG GAT CAA AGT GAG GCG |
| Chemicals, Enzymes and other reagents | ||
| Mouse Diet, High Fat Fat Calories (60%), Soft Pellets | Bio-Serv | S3282 Pellets |
| Thermo Scientific Chemicals Methylene Blue, pure, certified | ThermoFisherScientific | A42771AP |
| Oil Red O | ThermoFisherScientific | AAA1298914 |
| Aspartate Aminotransferase Reagent Kit | ThermoFisherScientific | 23-666-121 |
| Alanine Aminotransferase Reagent | ThermoFisherScientific | 23-666-089 |
| Pointe Scientific Cholesterol Liquid Reagents | ThermoFisherScientific | 23-666-200 |
| Cholesterol Standard | ThermoFisherScientific | SB-1012-030 |
| Pointe Scientific Triglycerides Liquid Reagents | ThermoFisherScientific | 23-666-410 |
| BODIPY™ 581/591 C11 (Lipid Peroxidation Sensor) | ThermoFisherScientific | D3861 |
| Oleic acid | MilliporeSigma | O1383-5G |
| Dimethyl 2-oxoglutarate | MilliporeSigma | 13192-04-6 |
| Pointe Scientific Glucose (Hexokinase) Liquid Reagents | ThermoFisherScientific | 23-666-278 |
| UC-514321 | MedChemExpress | HY-120395 |
| Mouse Insulin ELISA Kit | ThermoFisherScientific | Invitrogen™ EMINS |
| Software | ||
| R-studio | Posit | RStudio 2024.04.1 + 748 |
| GraphPad Prism | Dotmatics | Version 10.4.1 |