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. 2025 May 16;11(20):eado3440. doi: 10.1126/sciadv.ado3440

Fig. 2. Ppp1r3bΔhep mice develop glucose intolerance and steatosis with aging or sucrose diet challenge, while Ppp1r3bOE mice have increased liver glycogen and reduced TG content.

Fig. 2.

(A to E) Metabolic tests performed on fasted 6- to 9-month-aged Ppp1r3bf/f (black, n = 6) and Ppp1r3bΔhep (pink, n = 8) mice maintained on chow diet. (A) Intraperitoneal (ip) GTT [area under the curve (AUC) inset]. (B) ITT, AUC inset. (C) Hepatic glycogen content. (D) hepatic TG content. (E) Oil Red O staining. (F to I) Metabolic tests performed on fasted 6- to 9-month-aged Ppp1r3bf/f (black, n = 8) and Ppp1r3bhepOE (green, n = 6) mice maintained on chow diet. (F) Intraperitoneal GTT, AUC inset. (G) ITT, AUC inset. (H) Hepatic glycogen content. (I) Hepatic TG content. (J to N) Metabolic tests performed on fasted Ppp1r3bf/f (black, n = 5) and Ppp1r3bΔhep (pink, n = 7) mice on HSD (65%) for 12 weeks. (J) Intraperitoneal GTT, AUC inset. (K) ITT, AUC inset. (L) Hepatic glycogen content. (M) hepatic TG content. (N) Oil Red O staining. (O to S) Metabolic tests performed on fasted Ppp1r3bf/f (black, n = 8) and Ppp1r3bhepOE (green, n = 8) on HSD for 12 weeks. (O) Intraperitoneal GTT, AUC inset. (P) ITT, AUC inset. (Q) Hepatic glycogen content. (R) Hepatic TG content. (S) Oil Red O staining. Group average presented as bar + SD with individual animals represented as shapes. n.d., none detected.