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. 2025 May 16;20(5):e0318964. doi: 10.1371/journal.pone.0318964

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© 2025 Bean et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — A) The consensus sequence logo for the top 303 Tn916 insertion sites in wild type cells. The consensus sequence is similar to that determined for Tn916 insertions in other organisms [29,30]. B) Tn916 insertions in the hotspot between ykuC and ykyB (kre), where 22% of insertions occur. Insertions in the reverse (top strand) or forward (bottom strand) are indicated by bar plots, with additional insertion positions present at frequencies too low to plot to scale indicated by asterisks. A 17-bp perfect inverted repeat is indicated by the arrows below the sequence and gray shading. C) Sequence of the Tn916 insertion hotspot in C. difficile strain CD37 [45,46] and in C. difficile strain 630 [30]. Shading and arrows indicate the 8 bp inverted repeat in the hotspot in CD630.