Reagents and tools table

Reagent/resource	Reference or source	Identifier or catalog number
Experimental models
A431 cells (H. sapiens)	ATCC	Cat# CRL-1555, RRID:CVCL_0037
Human primary macrophages	Finnish Red Cross
A431 NUP93-GFP	Li et al, 2024	PMID: 38409044
A431 SOAT1-GFP oe	this study
A431 SOAT1-GFP oe NE-trap	this study
A431 SOAT1-SNAPf	this study
Recombinant DNA
pDendra2-C1	Prof. Vladislav Verkhusha, Albert Einstein College of Medicine
Antibodies
p65	Abcam	AB16502
DGAT1	Santa Cruz	sc-271934
anti-rabbit IgG (H + L) secondary antibody, Alexa Fluor™ 647	Thermo Fisher	A31573
SOAT1	Santa Cruz	sc-137013
Oligonucleotides and other sequence-based reagents
SUN2 cDNA	https://www.ncbi.nlm.nih.gov/nuccore/	NM_015374.3
NucleoSpin RNA isolation kit	Macherey-Nagel	Cat. 740955
Chemicals, Enzymes and other reagents
DAPI	Sigma-Aldrich	D9542
DGAT1 inhibitor	Sigma-Aldrich	PZ0207
DGAT2 inhibitor	Sigma-Aldrich	PZ0233
SOAT1 inhibitor	Sigma-Aldrich	Sandoz 58-035
Fibronectin	Roche	11080938001
LD540	Princeton BioMolecular Research
TNFα	Sigma-Aldrich	T0157
TNFα	RnD Systems	210-TA
methyl-β-cyclodextrin	Sigma-Aldrich	C4555
cholesterol	Sigma-Aldrich	C8667
NucRed Live 647	Molecular Probes Life Technologies	R37106
glutaraldehyde	Sigma-Aldrich
oleic acid	Sigma-Aldrich,I	O1008
SNAP-Cell 647-Sir	New England Biolabs®	S9102
Software
Matlab R2020a	https://mathworks.com/products/matlab.html	RRID: SCR_001622
R 4.4.0	https://www.r-project.org/	RRID: SCR_001905
BIAS	Single-Cell Technologies Ltd https://single-cell-technologies.com/bias-2/
Object Analyzer	https://bitbucket.org/szkabel/lipidanalyser/downloads/
Microscopy image browser	https://mib.helsinki.fi/	RRID: SCR_016560
Other
SRS microscope	Tomberg et al, 2024	10.1364/OPTCON.532676