Table 1.
PCR information used in this study.
| Loci | PCR primers | Primer sequence (5’ – 3’) | PCR cycles | References |
|---|---|---|---|---|
| ITS | ITS5 | GGA AGT AAA AGT CGT AAC AAG G | 95 °C 5 min; (95 °C: 30 s, 55 °C: 30 s, 72 °C: 1 min) × 35 cycles; 72 °C 10 min | White et al. (1990) |
| ITS4 | TCC TCC GCT TAT TGA TAT GC | |||
| LSU | LR0R | GTA CCC GCT GAA CTT AAG C | 95 °C 5 min; (94 °C: 30 s, 52 °C: 45 s, 72 °C: 1.5 min) × 30 cycles; 72 °C 10 min | Hurdeal et al. (2023) |
| LR5 | TCC TGA GGG AAA CTT CG | |||
| TEF1α | EF1-983F | GCYCCYGGHCAYCGTGAYTTYAT | 95 °C 5 min; (95 °C: 30 s, 55 °C: 60 s, 72 °C: 60 s) × 30 cycles; 72 °C 10 min | Jaklitsch et al. (2005) |
| TEF1LLErev | AACTTGCAGGCAATGTGG | |||
| Act | ACT-1 | TGG GAC GAT ATG GAI AAI ATC TGG CA | 95 °C 3 min; (95 °C: 60 s, 55 °C: 60 s, 72 °C: 1 min) × 30 cycles; 72 °C 10 min | Voigt and Wöstemeyer (2000) |
| ACT-4R | TC ITC GTA TIC TIG CTI IGA IAT CCA CA T | |||
| SSU | NS1 | GTA GTC ATA TGC TTG TCT CC | 95 °C 5 min; (94 °C: 60 s, 54 °C: 50 s, 72 °C: 1 min) × 37 cycles; 72 °C 10 min | (White et al. 1990) |
| NS4 | CTT CCG TCA ATT CCT TTA AG |