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. 2025 May 19;26(4-6):e70008. doi: 10.1111/tra.70008

FIGURE 4.

FIGURE 4

BicD increases the fraction of processively moving kinesin‐1 motors. (A) Kymographs illustrating the trajectories of (left) kinesin or (right) the kinesin‐BicDCC2‐CC3 complex on MTs. (B) Recruitment of kinesin (motors/s/μm/μM) (0.32 ± 0.17, n = 42 MTs), the kinesin‐BicDCC2‐CC3 complex (0.50 ± 24, n = 41 MTs) and kinesinΔH2 (0.67 ± 0.24, n = 33 MTs) to the MT. Mean ± SD, N = 3 experiments. One‐way ANOVA with Tukey's multiple comparison, p values indicated on figure. (C) Percentage of bound motors that move processively (green), diffusively (purple) or that were static (gray). The percentage of processive events were 28% for kinesin, 52% for the kinesin‐BicDCC2‐CC3 complex and 48% for kinesinΔH2. (D) Speed distributions of kinesin (0.25 ± 0.19 μm/s, n = 74), the kinesin‐BicDCC2‐CC3 complex (0.33 ± 0.19, n = 84) and kinesinΔH2 (0.43 ± 0.25 μm/s, n = 111). Mean ± SD, N = 3 experiments. One‐way ANOVA with Tukey's multiple comparison, p values indicated on figure. (E) Run length of kinesin (1.2 ± 0.1 μm, n = 34), the kinesin‐BicDCC2‐CC3 complex (2.3 ± 0.1 μm, n = 63) and kinesinΔH2 (1.9 ± 0.1 μm, n = 79). N = 3 experiments. One‐way ANOVA with Tukey's multiple comparison, p values indicated on figure.