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. 2005 Aug 12;5:63. doi: 10.1186/1471-2334-5-63

Figure 2.

Figure 2

Primer and template sequence homology. Bacterial species are followed by their GenBank accession numbers. Primer sequences are given below the primer's name at the top, primary primers are in bold, nested primers are in italics and sequence shared by two primers is underlined. A dot (.) below each nucleotide in the primer represents homology at that position in each species' DNA with the primer and letter signifies the diverging nucleotide found in the bacteria DNA. A dash (-) represents spaces placed in the sequence for alignment purposes. Three asterisks (***) signifies the variable length region of DNA found between the forward and reverse primers and the number given at that site for each species indicates the size of the amplicons generated from the nested reaction.