Table 2.
List of primers (forward and reverse) used for DNA amplification of the partial mitochondrial (cox1) and ribosomal genes (18S and 28S rDNA) of parasitic ergasilids (Cyclopoida: Ergasilidae) found in the nostrils of fishes from Pardo River, São Paulo state, Brazil – including sequences and references
| Gene regions | Primers | Sequences | References |
|---|---|---|---|
| Cox1 | LCO1490 | 5-GGTCAACAAATCATAAAGATATTGG-3′ | Folmer et al. (1994) |
| HCO2198 | 5-TAAACTTCAGGGTGACCAAAAAATCA-3′ | ||
| 18S | 18SF | 5-AAGGTGTGMCCTATCAACT-3′ | Song et al. (2008) |
| 18SR | 5-TTA CTTCCTCTAAACGCTC-3′ | ||
| 28S | 28SF | 5-ACAACTGTGATGCCCTTAG-3′ | Song et al. (2008) |
| 28SR | 5-TGGTCCGTGTTTCAAGACG-3′ |