Figure 2.

Effect of HSPG on TNF-α release by murine microglia. In panel A, time course of TNF-α production by murine microglia in response to HSPG. Microglial cells were cultured in 96-well plates and stimulated with 30 μg/ml HSPG for up to 24 h. In panel B, dose-dependent effect of HSPG on the accumulation of TNF-α by murine microglia. Microglial cells were cultured in 96-well plates and stimulated for 24 h with increasing concentrations of HSPG or 100 ng/ml LPS. Mean values ± SD of assays performed with culture media supernatants collected and pooled from triplicate wells for each condition are shown (n = 9). Both panels depict a representative experiment out of three performed with similar results. **p < 0.01.