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Effect of HSPG on iNOS and TNF-α mRNA expression in murine microglia. One-day-old microglial cells were cultured in 48-well plates and then stimulated for 4 hours with 15 μg/ml HSPG or 100 ng/ml LPS. Total RNA was extracted and analysed by RT-PCR. The densitometric evaluation of the bands obtained from three independent experiments in duplicates are reported (n = 6); values are expressed as the relative level of iNOS/GAPDH and TNFα/GAPDH. *p < 0.05.
