Figure 4.

Effect of heat denaturated HSPG and HS-GAG chains on NO₂^- and TNF-α release by murine microglia. Microglial cells were cultured in 96-well plates and stimulated with 15 μg/ml HSPG, 15 μg/ml heat denaturated HSPG (HSPG-hd) or 15 μg/ml HS-GAG chains (HS). After 24 h, culture media supernatants were assayed for NO₂^- accumulation (A) and TNF-α release (B). Mean values ± SD of assays performed with culture media supernatants collected and pooled from triplicate wells for each condition are shown (n = 9). Both panels depict a representative experiment out of three performed with similar results. *p < 0.05, **p < 0.01.