Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Oligonucleotide Primers Used for cDNA amplification
| Probe | Cycles | Orientation | Tann | Sequence | Length of PCR Fragments (bp) |
| GAPDH | 20 | sense antisense |
68 | 5'TGAAGGTCGGTGTGAACGGATTTGGC3' 5'CATGTAGGCCATGAGGTCCACCAC3' |
983 |
| iNOS | 26 | sense antisense |
68 | 5'CCCTTCCGAAGTTTCTGGCAGCAGC3' 5'GGCTGTCAGAGCCTCGTGGCTTTGG3' |
493 |
| TNFα | 24 | sense antisense |
60 | 5'TTCTGTCTACTGAACTTCGGGTGATCGGTCC3' 5'GTATGAGATAGCAAATCGGCTGACGGTGTGGG3' |
378 |
