Table 3. Expected mass of the NPM2-H1.8-GFP complex.

Sucrose gradient elution volume indicates that the NPM2-H1.8-GFP complex is smaller than mono-nucleosome (around 230 kDa). Only the NMP2 pentamer complexed H1.8-GFP monomer (166 kDa) reasonably explains the sucrose gradient result.

Name	Molecular wight (Da)
NPM2 monomer	21,917
NPM2 pentamer	109,587
NPM2 decamer	219,175
H1.8-GFP	56,704
NPM2 pentamer +H1.8 GFP monomer	166,291
NPM2 pentamer +H1.8 GFP pentamer	393,107
NPM2 decamer +H1.8 GFP monomer	275,878
NPM2 decamer +H1.8 GFP pentamer	502,694
Nucleosome (193 bp DNA)	228,111