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. 2025 May 20;108(2):00368504251343177. doi: 10.1177/00368504251343177

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© The Author(s) 2025

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 3. — Downregulation of αB-crystallin enhances radiation-induced apoptosis in basal-like breast cancer cells. (a)–(d) The apoptosis rate was estimated using flow cytometry in MDA-MB-231 + shαB, MCF-7 + OE-αB, and control cells at 48 h after exposure to 4 Gy of X-rays. Data are represented as means ± SD (n = 6; unpaired two-tailed Student's t-test; **p < 0.01). (e)–(h) Western blot analysis was performed to evaluate the expression levels of proteins associated with apoptosis at 48 hours after 4 Gy of X-ray irradiation. The histogram presents the results of the quantitative analysis of the protein bands. Data are represented as means ± SD (n = 3; unpaired two-tailed Student's t-test; *p < 0.05, **p < 0.01). OE-αB: cells transfected with αB-crystallin overexpression vectors; shαB: cells transfected with αB-crystallin-specific shRNA; shNC and OE-NC: cells transfected with corresponding empty vectors.