Table 2.

Gene primers used in the molecular characterization of R. Equi isolates

Gene	Primers 1	Sequence	Size (bp)	Reference
choE	COX-F	GTCAACAACATCGACCAGGCG	959	[67]
	COX-R	CGAGCCGTCCACGACGTACAG
vapA	VAPA-F	ACAAGACGGTTTCTAAGGCG	550	[46]
	VAPA-R	TTGTGCCAGCTACCAGAGCC
vapB	VAPB-F	GAATTCGAAAGCGCAAAGGT	650	[46]
	VAPB-R	TTCCGTGAACATCGTACTGC
vapC	VAPC-F	GGGTCGTCCATCCAAATCGA	700	[46]
	VAPC-R	GGTCAGGCCTATCACCCTTG
vapD	VAPD-F	GGTGGTGCGATGTCAGAATG	400	[46]
	VAPD-R	TGGAACGTCTTGCCCTTCTT
vapE	VAPE-F	ATATGACGACCGTTCACAAG	600	[46]
	VAPE-R	CTCCGATGCCCACCAAACTA
vapF	VAPF-F	AGAATATGCCTGGTATGGGC	350	[46]
	VAPF-R	TCGTCGTATAGCTGCTGCAG
vapH	VAPH-F	AATTCCTATCAAGGACAGC	500	[46]
	VAPH-R	ATACCGATTACGGAGCTCAC
vapN	VAPN-F	GGTACTGCAGGCAACTGCTA	425	[45]
	VAPN-R	GAGCTGCTACTACCGTGGTC
traA	TRAA-F	AGAGTTCATGCGTGACAACG	959	[68]
	TRAA-R	GTCCACAGGTCACCGTTCTT
16 S rRNA	27-F	AGAGTTTGATCCTGGCTCAG	1350	[69]
	1492-R	GGTTACCTTGTTACGACTT

¹ Each primer for each gene was tested in a uniplex PCR