Table 5.

Cutoff points and interpretation ranges for each measurement moment of the biofilm formation assay

Rating a	Interpretation ranges
	24 h	48 h	72 h
Cutoff	0.053	0.066	0.069
Non-producer	≤ 0.053	≤ 0.066	≤ 0.069
Weak	0.053 < Odt ≤ 0.106	0.066 < Odt ≤ 0.132	0.069 < Odt ≤ 0.138
Moderate	0.106 < Odt ≤ 0.212	0.132 < Odt ≤ 0.264	0.138 < Odt ≤ 0.276
Strong	Odt > 0.212	Odt > 0.264	Odt > 0.276

^a The cutoff point for each measurement was calculated based on the standard deviation of the OD values of the negative control wells (C-) and then multiplied by three and added to the average C- to obtain it. The values of the classification ranges of the isolates varied according to the cutoff point measured on each day of the test, calculated in accordance with what was described in the methodology