| Genetic reagent |
pJV53 |
van Kessel and Hatfull, 2007
|
pJV53 plasmid from Dr. Hatfull’s lab wherein the kanr antibiotic cassette was swapped with aprar cassette. |
aprares
|
| Genetic reagent |
pSTKiToff
|
Soni et al., 2015
|
L5 integrative pST-KiT plasmid harboring reverse tetR gene. |
kanres
|
| Genetic reagent |
pST-wag31 |
|
pST-KiToff construct harboring wag31 between NdeI-HindIII sites. |
kanres
|
| Genetic reagent |
pST-gfp |
|
pST-KiToff construct harboring gfp between NdeI-HindIII sites. |
kanres
|
| Genetic reagent |
pET-wag31 |
|
pET28b harboring His-wag31-His |
kanres
|
| Genetic reagent |
pET-wag31K20A
|
|
pET28b harboring His-wag31K20A-His |
kanres
|
| Genetic reagent |
pET-wag31∆N
|
|
pET28b harboring His-wag31Δ1-60-His |
kanres
|
| Genetic reagent |
pET-wag31-gfp |
|
pET28b harboring His-wag31-gfp-His |
kanres
|
| Genetic reagent |
pSCG-wag31 |
|
Giles integrative plasmid harboring His-wag31-His under the control of wag31 native promoter |
aprares
|
| Genetic reagent |
pSCG-wag31K20A
|
|
Giles integrative plasmid harboring His-wag31K20A-His under the control of wag31 native promoter |
aprares
|
| Genetic reagent |
pSCG-wag31∆N
|
|
Giles integrative plasmid harboring His- wag31ΔN-His under the control of wag31 native promoter |
aprares
|
| Genetic reagent |
pNit-wag31 |
|
IVN inducible plasmid harboring 3X-FLAG-wag31 |
kanres
|
| Genetic reagent |
pNit-murG |
|
IVN inducible plasmid harboring 3X-FLAG-murG |
kanres
|
| Genetic reagent |
pNit-sepIVA |
|
IVN inducible plasmid harboring 3X-FLAG-sepIVA |
kanres
|
| Genetic reagent |
pNit-msm2092 |
|
IVN inducible plasmid harboring 3X-FLAG-msm2092 |
kanres
|
| Genetic reagent |
pNit-accA3 |
|
IVN inducible plasmid harboring 3X-FLAG-accA3 |
kanres
|
| Genetic reagent |
pNit-mmpS5 |
|
IVN inducible plasmid harboring 3X-FLAG-mmpS5 |
kanres
|
| Genetic reagent |
pNit-mmpL4 |
|
IVN inducible plasmid harboring 3X-FLAG-mmpL4 |
kanres
|
| Gene (Mycobacterium smegmatis) |
wag31 |
Mycobrowser |
Uniprot A0R006
|
|
| Gene (M. smegmatis) |
murG |
Mycobrowser |
Uniprot A0R016
|
|
| Gene (M. smegmatis) |
sepIVA |
Mycobrowser |
Uniprot A0QV18 |
|
| Gene (M. smegmatis) |
Msm2092 |
Mycobrowser |
Uniprot A0QU64 |
|
| Gene (M. smegmatis) |
accA3 |
Mycobrowser |
Uniprot A0QTE1 |
|
| Gene (M. smegmatis) |
mmpS5 |
Mycobrowser |
Uniprot A0QP03 |
|
| Gene (M. smegmatis) |
mmpL4 |
Mycobrowser |
Uniprot A0QY12 |
|
| strain, strain background (M. smegmatis) |
Δwag31 |
This study |
|
Hygres, kanres, |
| Strain, strain background (M. smegmatis) |
Msm::gfp |
This study |
|
kanres, |
| Strain, strain background (M. smegmatis) |
Δwag31::wag31 |
This study |
|
Hygres, kanres, apramycinres
|
| Strain, strain background (M. smegmatis) |
Δwag31::wag31K20A
|
This study |
|
Hygres, kanres, apramycinres
|
| Strain, strain background (M. smegmatis) |
Δwag31::wag31ΔN
|
This study |
|
Hygres, kanres, apramycinres
|
| Strain, strain background (M. smegmatis) |
Δwag31::wag31ΔC
|
This study |
|
Hygres, kanres, apramycinres
|
| Strain, strain background (M. smegmatis) |
Msm::wag31 |
This study |
|
Kanres
|
| Strain, strain background (M. smegmatis) |
Msm::murG |
This study |
|
Kanres
|
| Strain, strain background (M. smegmatis) |
Msm::sepIVA |
This study |
|
Kanres
|
| Strain, strain background (M. smegmatis) |
Msm::msm2092 |
This study |
|
Kanres
|
| Strain, strain background (M. smegmatis) |
Msm::accA3 |
This study |
|
Kanres
|
| Strain, strain background (M. smegmatis) |
Msm::mmpS5 |
This study |
|
Kanres
|
| Strain, strain background (M. smegmatis) |
Msm::mmpL4 |
This study |
|
Kanres
|
| Genetic reagent (E. coli) |
E. coli DH5α |
Invitrogen |
|
Used for clonings |
| Genetic reagent (E. coli) |
E. coli BL21 DE3 |
Novagen |
|
Used for protein expression |
| Chemical compound |
BODIPY |
Invitrogen |
D3922 |
Fluorescent stain |
| Chemical compound |
Acridine orange 10-Nonyl bromide (NAO) |
Invitrogen |
A1372 |
Fluorescent stain |
| Chemical compound |
2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) |
Avanti polar lipids |
850375 C |
Lipid used for PLiMAP |
| Chemical compound |
2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) |
Avanti polar lipids |
850725 C |
Lipid used for PLiMAP |
| Chemical compound |
1’,3’-bis(1,2-dioleoyl-sn-glycero-3-phospho)-glycerol (DOPG) |
Avanti polar lipids |
840475 C |
Lipid used for PLiMAP |
| Chemical compound |
FAST Dil |
Invitrogen |
D7756 |
Lipid used for tethering assay |
| Chemical compound |
FAST DiO |
Invitrogen |
D3898 |
Lipid used for tethering assay |
| Chemical compound |
Cardiolipin |
Avanti polar lipids |
841199 C |
Lipid used for PLiMAP |
| Chemical compound |
Cobalt agarose beads |
GoldBio |
H-310–5 |
Resin used for His-Pull down assays |
| Chemical compound |
FLAG M2 Magnetic beads |
Sigma |
M8823 |
Resin used for Immunoprecipitation |
| Chemical compound |
TALON Metal affinity resin |
Takara Bio |
635653 |
Resin used for Protein purification |
| Chemical compound |
Ni-NTA Agarose beads |
Qiagen |
30210 |
Resin used for Protein purification |
