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. 2025 May 23;8:0708. doi: 10.34133/research.0708

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Copyright © 2025 Tianlong Huang et al.

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Fig. 9. — Visualization of the combination effects of GNS561 with cisplatin as a dual drug regimen. (A) Confidence interval graph for a pair of synergistic drugs (cisplatin and GNS561). (B to E) Synergy plots for the combined treatment of U2OS/R cells with cisplatin and GNS561 using Bliss (B), HSA (C), Loewe (D), and ZIP (E) methods. Synergy scores were calculated using the SynergyFinder software. Positive or negative synergy scores indicate synergistic or antagonistic effects, respectively. (F) Synergy measurement for a given dose combination of cisplatin with 1.5 μM GNS561. (G) Plate cloning formation experiment based on the recommended concentrations from the barometer in (F). (H) Flow cytometry and caspase-3 activity assays evaluating the apoptotic response to GNS561 and cisplatin combination treatment in MG63/R and U2OS/R cells. (I to K) Representative tumor images (I), tumor growth curves (J), and tumor weights (K) from nude mice across different treatment groups. ***P < 0.001.