Table 3.
Primer sequence of real-time quantitative PCR.
| Gene | Forward (5′ to 3′) | Reverse (5′ to 3′) | Amplicon, bp | Primer efficiency | Accession No. |
|---|---|---|---|---|---|
| β-Actin | GATGATGAAATTGCCGCACTG | ACCGACCATGACGCCCTGATGT | 135 | 0.95 | M25013 |
| gpx1 | AGGGGCTGGTTATTCTGGG | GGGCGTTCTCACCATTCAC | 162 | 0.99 | EU828796.2 |
| gpx4 | TACGCTGAGAGAGGTTTACAC | CTTTTCCATTGGGTTGTTCC | 196 | 0.94 | KU255598 |
| Gsto | GGTGCTCAATGCCAAGGGAA | CTCAAACGGGTCGGATGGAA | 208 | 1.02 | KT757314 |
| Gr | GTGTCCAACTTCTCCTGTG | ACTCTGGGGTCCAAAACG | 221 | 0.98 | JX854448 |
| Cat | GAAGTTCTACACCGATGAGG | CCAGAAATCCCAAACCAT | 157 | 1.03 | FJ560431 |
| Cuznsod | CGCACTTCAACCCTTACA | ACTTTCCTCATTGCCTCC | 218 | 0.99 | GU901214 |
| Mnsod | ACGACCCAAGTCTCCCTA | ACCCTGTGGTTCTCCTCC | 111 | 0.93 | GU218534 |
| nrf2 | CTGGACGAGGAGACTGGA | ATCTGTGGTAGGTGGAAC | 234 | 1.05 | KF733814 |
| keap1 | TTCCACGCCCTCCTCAA | TGTACCCTCCCGCTATG | 205 | 1.03 | KF811013 |
| il-1β | AGAGTTTGGTGAAGAAGAGG | TTATTGTGGTTACGCTGGA | 234 | 0.96 | JQ692172 |
| il-6 | CAGCAGAATGGGGGAGTTATC | CTCGCAGAGTCTTGACATCCTT | 134 | 0.99 | KC535507.1 |
| tnf-α | ACGCTCAACAAGTCTCAG | CTGGCTGTAGACGAAGTAA | 252 | 1.01 | HQ696609 |
| nf-κb | GAAGAAGGATGTGGGAGATG | TGTTGTCGTAGATGGGCTGAG | 124 | 1.04 | AC131025.1 |
| Occludin | TATCTGTATCACTACTGCGTCG | CATTCACCCAATCCTCCA | 208 | 0.93 | KF193855.1 |
| Claudin12 | CCCTGAAGTGCCCACAA | GCGTATGTCACGGGAGAA | 81 | 1.01 | KF998571 |
| zo-1 | CGGTGTCTTCGTAGTCGG | CAGTTGGTTTGGGTTTCAG | 154 | 0.97 | KF193852.1 |
| zo-2 | TACAGCGGGACTCTAAAATGG | TCACACGGTCGTTCTCAAAG | 150 | 0.99 | KM112095 |
β-actin = beta-actin; gpx1 = glutathione peroxidase 1; gpx4 = glutathione peroxidase 4; gsto = glutathione S-transferase omega; gr = glutathione reductase; cat = catalase; cuznsod = copper/zinc superoxide dismutase; mnsod = manganese superoxide dismutase; nrf2 = nuclear factor erythroid 2-related factor 2; keap1 = kelch-like ECH-associated protein 1; il-1β = interleukin-1β; il-6 = interleukin-6; tnf-α = tumor necrosis factor alpha; nf-κb = nuclear factor kappa-B; zo-1 = zonula occludens-1; zo-2 = zonula occludens-2.