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. 2025 May 13;16(24):11110–11122. doi: 10.1039/d5sc01863c

Table 1. CYP125MRCA dissociation (Kd) constants (μM) for various steroid substrates using the Hill modela.

Substrate K d (μM) (n*)
Cholest-4-en-3-one 0.85 ± 0.02 (2.15)
Cholesterol 2.00 ± 0.05 (4.45)
Sitosterol 1.48 ± 0.03 (3.74)
Stigmast-4-en-3-one 1.61 ± 0.11 (1.98)
Stigmasterol 2.04 ± 0.12 (2.36)
Campesterol 4.19 ± 0.05 (3.68)
Cholecalciferol (VD3) 1.60 ± 0.22 (1.84)
a

*n is the Hill coefficient as shown in the Hill equation (eqn (1)).