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. 2025 May 12;73(20):12132–12145. doi: 10.1021/acs.jafc.4c12140

2. Compositional Analysis of Ryegrass (RG) Used in all Three Experiments and Plantain (PL) Incubated in Experiment 1.

substrates OMD (% DM) NDF (% DM) ADF (% DM) lignin (% DM) lignin/NDF % ash (% DM) SSS (% DM) CP (% DM) crude fat (% DM) NSC (% DM) NSC/CP plantain% acteoside (mg/g pasture DM) aucubin (mg/g pasture DM) catalpol (mg/g pasture DM)
ryegrass (RG) 83.2 42.8 21.3 6.3 14.7 12.6 5.8 27.2 3.8 13.6 0.5 n.d n.d n.d n.d
plantain (PL) substrates PL1 70.0 41.5 25.5 8.7 21.0 11.9 6.2 16.8 2.5 27.2 1.6 45.3 9.9 9 0.9
PL2 68.5 42.7 27.5 8.3 19.4 11.5 7.5 16.6 2.5 26.8 1.6 48.0 18.3 5.8 0.5
PL3 67.3 43.8 26.3 8.4 19.2 11.8 6.3 17.7 2.2 24.6 1.4 56.5 6.6 9.9 0.6
a

Plantain percentage in the substrate.

b

RG value was over two interquartile ranges compared to the averages of PL1, PL2, and PL3 within columns, n.d: not detected.

c

Plantain substrate (PL1) containing 10 mg of acteoside/g pasture DM and 9 mg aucubin/g pasture DM.

d

Plantain substrate (PL2) containing 18 mg acteoside/g pasture DM and 6 mg aucubin/g pasture DM.

e

Plantain substrate (PL3) containing 6 mg acteoside/g pasture DM and 9 mg aucubin/g pasture DM, DM: dry matter, OMD: organic matter digestibility in vivo, NDF: neutral detergent fiber, ADF: acid detergent fiber, SSS: soluble sugars and starch.