3. Gas and Methane (CH4) Production Parameters of Plantain (PL) Substrates Compared to Ryegrass (RG) Substrate In Vitro in Experiment 1 .
| substrates | GP at 12 h (mL/g DM) | GP at 24 h (mL/g DM) | PGP (mL/g DM) | CH4 at 12 h (mL/g DM) | CH4 at 24 h (mL/g DM) | PCH4 (mL/g DM) | CH4/GP at 12 h (%) | CH4/GP at 24 h (%) | PCH4/PGP (%) | T1/2 GP (h) | T1/2 CH4 (h) | R1/2 GP (mL/h) | R1/2 CH4 (mL/h) | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ryegrass | (RG) | 201.8 | 266.3 | 295.6 | 25.3 | 38.4 | 41.7 | 12.5 | 14.4 | 14.1 | 7.4 | 9.7 | 14.5 | 2.1 |
| plantain (PL) substrates | PL1 | 200.5 | 249.5 | 307.5 | 25.1 | 35.0 | 44.2 | 12.5 | 14.0 | 14.2 | 6.9 | 10.1 | 12.3 | 1.5 |
| PL2 | 204.2 | 254.2 | 311.6 | 24.4 | 35.4 | 42.6 | 11.9 | 13.9 | 13.6 | 6.8 | 10.0 | 12.6 | 1.6 | |
| PL3 | 188.3 | 240.3 | 305.2 | 23.3 | 33.4 | 45.1 | 12.3 | 13.8 | 14.6 | 7.9 | 12.0 | 10.7 | 1.3 | |
| SEM | 9.52 | 7.25 | 21.01 | 2.4 | 3.19 | 5.56 | 0.72 | 0.93 | 1.0 | 0.15 | 1.13 | 0.23 | 0.15 | |
| p-value | <0.01 | <0.01 | 0.18 | <0.01 | <0.01 | 0.5 | 0.02 | 0.09 | 0.59 | <0.01 | 0.17 | <0.01 | <0.01 | |
a
PGP: potential GP, PCH4: potential methane production, GP: gas production, CH4: methane production, h: hours, T 1/2: the half time required to reach the potential gas or methane production, R 1/2: the rate to produce half of potential gas or methane production, values with the same letter within the same columns are not significant (p > 0.05).
b
PL1: plantain substrate containing 10 mg of acteoside/g pasture DM and 9 mg aucubin/g pasture DM.
c
PL2: plantain substrate containing 18 mg acteoside/g pasture DM and 6 mg aucubin/g pasture DM.
d
PL3: plantain substrate containing 6 mg acteoside/g pasture DM and 9 mg aucubin/g pasture DM, SEM: standard error of means.