5. In Vitro gas and Methane (CH4) Production Parameters of Different Concentrations of PSM Tested in Experiment 2.
| substrates | GP at 12 h (mL/g DM) | GP at 24 h (mL/g DM) | PGP (mL/g DM) | CH4 at 12 h (mL/g DM) | CH4 at 24 h (mL/g DM) | PCH4 (mL/g DM) | CH4/GP at 12 h (%) | CH4/GP at 24 h (%) | PCH4/PGP (%) | T1/2 GP (h) | T1/2 CH4 (h) | R1/2 GP (mL/h) | R1/2 CH4 (mL/h) | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| control treatments | RG | 202.9 | 267.3 | 294.1 | 25.5 | 38.6 | 42.1 | 12.6 | 14.4 | 14.3 | 7.27 | 9.7 | 14.7 | 2.09 |
| RG-DMF | 206.7 | 269.5 | 296.4 | 25.2 | 37.6 | 40.4 | 12.1 | 13.9 | 13.6 | 7.06 | 9.6 | 15.0 | 2.13 | |
| acteoside treatments | ACT-6 | 206.8 | 272.5 | 299.1 | 25.1 | 38.6 | 42.4 | 12.0 | 14.1 | 14.1 | 7.25 | 10.1 | 15.0 | 2.08 |
| ACT-10 | 201.3 | 268.4 | 298.1 | 24.5 | 37.8 | 41.1 | 12.1 | 14.0 | 13.7 | 7.55 | 10.0 | 14.3 | 2.07 | |
| ACT-18 | 204.6 | 270.7 | 300.0 | 24.6 | 37.8 | 41.3 | 12.0 | 13. Nine | 13.7 | 7.39 | 10.0 | 14.5 | 2.05 | |
| ACT-36 | 203.5 | 269.6 | 295.8 | 23.5 | 36.3 | 39.7 | 11.5 | 13.4 | 13.4 | 7.37 | 10.1 | 14.9 | 1.98 | |
| aucubin treatments | AUC-6 | 204.5 | 272.1 | 300.7 | 24.8 | 38.2 | 41.9 | 12.1 | 14.0 | 13.9 | 7.48 | 10.1 | 14.7 | 2.06 |
| AUC-9 | 204.1 | 271.4 | 299.4 | 24.9 | 38.1 | 41.2 | 12.1 | 14.0 | 13.7 | 7.46 | 9.9 | 14.7 | 2.12 | |
| AUC-12 | 207.0 | 272.4 | 298.2 | 25.0 | 37.8 | 41.1 | 12.0 | 13.8 | 13.7 | 7.21 | 9.8 | 15.1 | 2.1 | |
| AUC-24 | 192.4 | 261.1 | 293.1 | 22.5 | 35.5 | 38.5 | 11.6 | 13.5 | 13.1 | 7.93 | 10.3 | 13.5 | 1.95 | |
| catalpol | CAT-10 | 192.7 | 256.4 | 287.6 | 23.0 | 35.7 | 39.9 | 11.8 | 13.9 | 13.8 | 7.58 | 10.4 | 13.6 | 1.89 |
| SEM | 10.01 | 8.98 | 13.5 | 2.98 | 3.78 | 4.37 | 0.91 | 0.98 | 0.91 | 0.34 | 0.35 | 0.49 | 0.17 | |
| p value | 0.02 | 0.08 | 0.62 | <0.01 | <0.01 | 0.07 | 0.07 | 0.01 | 0.01 | <0.01 | 0.55 | 0.01 | 0.26 | |
Ryegrass control.
Dimethylformamide added ryegrass control.
Treatments within the columns corresponding to varying doses of acteoside (denoted as ACT) and the numeric values separated by a hyphen indicating the amount of acteoside in mg/g of pasture DM.
Treatments within the columns corresponding to varying doses of aucubin (denoted as AUC) and the numeric values separated by a hyphen indicating the amount of aucubin in mg/g of pasture DM.
10 mg catalpol/g pasture DM.
The model was significant for GP at 12 h (p < 0.05), PGP: potential GP, PCH4: potential methane production, GP: gas production, CH4: methane production, h: hours, T 1/2: the half time required to reach the potential gas or methane production, and R 1/2: the rate to produce half of potential gas or methane production, but the mean comparisons were similar across all treatments, making them incomparable, values with the same letter within the same columns are not significant (p > 0.05), SEM: standard error of means.