6. Comparison of Short-chain Fatty Acids (SCFA) and Molar Proportion of SCFA Production In Vitro of Ryegrass (RG) Substrates Added with Aucubin (AUC-24), Acteoside (ACT-36), or Catalpol (CAT-10) in Experiment 2 over Time.
| molar
proportion (mol per 100 mol of total
SCFA)
|
ratios |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| substrates | SCFA | acetate | butyrate | propionate | valerate | caproate | iso-butyrate | iso-valerate | A/P | AB/PV |
| RG | 74.2 | 67.5 | 10.7 | 16.9 | 1.34 | 0.10 | 1.43 | 2.14 | 4.02 | 4.32 |
| RG-DMF | 70.6 | 66.5 | 10.6 | 17.6 | 1.35 | 0.12 | 1.55 | 2.20 | 3.78 | 4.08 |
| ACT-36 | 91.5 | 66.6 | 9.5 | 17.0 | 1.22 | 0.18 | 3.47 | 2.29 | 3.95 | 4.22 |
| AUC-24 | 84.6 | 66.6 | 9.8 | 17.5 | 1.11 | 0.10 | 3.07 | 2.14 | 3.87 | 4.17 |
| CAT-10 | 66.6 | 66.3 | 10.7 | 18.1 | 1.34 | 0.14 | 1.43 | 2.16 | 3.68 | 3.99 |
| SEM | 3.43 | 1.22 | 1.17 | 0.96 | 0.20 | 0.02 | 0.26 | 0.42 | 0.17 | 0.18 |
| treatments (Tr) | <0.01 | 0.99 | <0.01 | 0.42 | <0.01 | <0.01 | <0.01 | 0.75 | 0.51 | 0.58 |
| sampling time (Ti) | <0.01 | 0.53 | 0.95 | 0.12 | <0.01 | <0.01 | 0.85 | <0.01 | 0.42 | 0.66 |
| Tr × Ti | 0.25 | 0.42 | 0.44 | 0.76 | 0.96 | 0.47 | 0.32 | 0.75 | 0.7 | 0.74 |
a
Molar proportion of individual SCFAs.
b
Total short-chain fatty acids (mmol).
c
Acetate to propionate ratio (A/P).
d
[acetate + butyrate] to [propionate + valerate] ratio (AB/PV).
e
Ryegrass control.
f
Dimethylformamide added ryegrass control.
g
Ryegrass added with 36 mg acteoside/g pasture DM.
h
Ryegrass added with 24 mg aucubin/g pasture DM.
i
Ryegrass added with 10 mg catalpol/g pasture DM, values with the same letter within the same columns are not significant (p > 0.05), SEM: standard error of means.