8. In Vitro gas and Methane (CH4) Production Parameters of Treatments Containing Different Concentrations of PSM Added to Ryegrass (RG) Tested in Experiment 3.
| treatments | GP at 12 h (mL/g DM) | GP at 24 h (mL/g DM) | PGP (mL/g DM) | CH4 at 12 h (mL/g DM) | CH4 at 24 h (mL/g DM) | PCH4 (mL/g DM) | CH4/GP at 12 h (%) | 5CH4/GP at 24 h (%) | 6PCH4/PGP (%) | T1/2 GP (h) | T1/2 CH4 (h) | R1/2 GP (mL/h) | R1/2 CH4 (mL/h) | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| control | RG | 190.5 | 282.7 | 328.7 | 21.6 | 36.2 | 44.7 | 11.4 | 12.8 | 13.6 | 9.9 | 12.4 | 13.3 | 1.6 |
| acteoside treatments | ACT-50 | 204.4 | 297.5 | 342.7 | 22.2 | 37.5 | 46.8 | 10.8 | 12.6 | 13.7 | 9.5 | 12.7 | 14.3 | 1.7 |
| ACT-75 | 205.3 | 296.3 | 344.6 | 21.8 | 36.6 | 46.1 | 10.6 | 12.4 | 13.3 | 9.4 | 12.6 | 14.0 | 1.6 | |
| ACT-100 | 213.5 | 306.6 | 354.5 | 21.3 | 34.7 | 40.6 | 10.0 | 11.3 | 11.4 | 9.3 | 11.4 | 14.6 | 1.7 | |
| aucubin treatments | AUC-50 | 200.2 | 292.5 | 340.1 | 23.0 | 38.4 | 47.9 | 11.5 | 13.1 | 14.0 | 9.7 | 12.4 | 13.9 | 1.7 |
| AUC-75 | 198.0 | 294.9 | 350.2 | 22.2 | 37.7 | 47.3 | 11.2 | 12.8 | 13.4 | 10.2 | 12.7 | 13.5 | 1.7 | |
| AUC-100 | 187.3 | 286.8 | 360.5 | 20.4 | 36.4 | 50.4 | 10.9 | 12.7 | 13.9 | 11.5 | 14.8 | 12.2 | 1.5 | |
| combined treatment | ACT50+ AUC75 | 211.4 | 310.4 | 372.0 | 21.8 | 37.7 | 48.8 | 10.3 | 12.1 | 13.0 | 10.1 | 13.3 | 14.0 | 1.7 |
| ACT75+ AUC50 | 205.9 | 294.2 | 336.7 | 21.6 | 36.6 | 45.1 | 10.5 | 12.5 | 13.5 | 9.2 | 12.6 | 14.5 | 1.7 | |
| catalpol | CAT-50 | 200.0 | 298.94 | 353.7 | 21.8 | 38.0 | 48.7 | 10.9 | 12.7 | 13.6 | 10.23 | 13.2 | 13.8 | 1.7 |
| SEM | 4.72 | 5.23 | 11.75 | 1.05 | 2.88 | 5.13 | 0.53 | 0.9 | 1.06 | 0.53 | 0.89 | 0.55 | 0.12 | |
| p value | <0.001 | <0.001 | <0.001 | 0.004 | 0.025 | 0.042 | 0.01 | 0.03 | 0.021 | 0.005 | 0.007 | 0.004 | 0.035 | |
| contrasts, p values | ||||||||||||||
| ACT50+AUC75 vs (ACT-50 + AUC-75)/2 | 0.008 | 0.011 | 0.021 | 0.432 | 0.855 | 0.420 | <0.0001 | <0.0001 | 0.043 | 0.267 | 0.068 | 0.428 | 0.214 | |
| ACT-50 vs AUC-75 | 0.170 | 0.702 | 0.516 | 0.707 | 0.767 | 0.789 | 0.002 | 0.149 | 0.528 | 0.011 | 0.900 | 0.001 | 0.996 | |
| ACT75 + AUC50 vs (ACT-75 + AUC-50)/2 | 0.364 | 0.999 | 0.619 | 0.092 | 0.241 | 0.314 | 0.005 | 0.174 | 0.528 | 0.266 | 0.920 | 0.029 | 0.624 | |
| ACT-75 vs AUC-50 | 0.231 | 0.623 | 0.862 | 0.057 | 0.072 | 0.403 | 0.001 | 0.005 | 0.119 | 0.588 | 0.781 | 0.688 | 0.016 | |
Ryegrass control.
Treatments within the rows corresponding to varying doses of acteoside (denoted as ACT), and the numeric values separated by a hyphen indicating the amount of acteoside in mg/g of pasture DM.
Treatments within the rows corresponding to varying doses of aucubin (denoted as AUC), and the numeric values separated by a hyphen indicating the amount of aucubin in mg/g of pasture DM.
50 mg acteoside/g pasture DM added with 75 mg aucubin/g pasture DM.
75 mg acteoside/g pasture DM added with 50 mg aucubin/g pasture DM.
50 mg catalpol/g pasture DM.
p value of planned contrasts in selected treatments, PGP: potential GP, PCH4: potential methane production, GP: gas production, CH4: methane production, h: hours, T 1/2: the half time required to reach the potential gas or methane production, R 1/2: the rate to produce half of potential gas or methane production, values with the same letter within the same columns are not significant (p > 0.05), SEM: standard error of means.