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. 2025 May 9;13:1535381. doi: 10.3389/fpubh.2025.1535381

Table 3.

The detection performance of STSS and NIPT for Down syndrome in different maternal age groups.

Description of parameters NIPT performance STSS performance
All Maternal age < 35 years Maternal age ≥ 35 years All Maternal age < 35 years Maternal age ≥ 35 years
TP 163 100 63 83 33 50
FP 35 28 7 11,218 7,265 3,953
TN 140,062 120,522 19,540 128,879 113,285 15,594
FN 5 3 2 85 70 15
Sensitivity
(95% CI)
97.02%
(94.45–99.59%)
97.09%
(93.84–100%)
96.92%
(92.73–100%)
49.41%
(41.85–56.97%)
32.04%
(23.02–41.05%)
76.92%
(66.68–87.17%)
Specificity
(95% CI)
99.98%
(99.97–99.98)
99.98%
(99.97–99.99%)
99.96%
(99.94–99.99%)
91.99%
(91.85–92.14%)
93.97%
(93.84–94.11%)
79.78%
(79.21–80.34%)
PPV
(95% CI)
82.32%
(77.01–87.64%)
78.13%
(70.96–85.29%)
90.00%
(82.97–97.03%)
0.73%
(0.58–0.89%)
0.45%
(0.30–0.61%)
1.25%
(0.91–1.59%)
NPV
(95% CI)
99.99%
(99.99–100%)
99.99%
(99.99–100%)
99.99%
(99.98–100%)
99.93%
(99.92–99.95%)
99.94%
(99.92–99.95%)
99.90%
(99.86–99.95%)
FPR 0.03% 0.02% 0.04% 8.01% 6.03% 20.22%
FNR 2.98% 2.91% 3.08% 50.60% 67.96% 23.08%

CI, confidence interval; PPV, positive predictive value; NIPT, non-invasive prenatal testing; TP, true positives; NPV, negative predictive value; STSS, second-trimester serum screening; FP, false positives; FPR, false positive rate; TN, true negatives; FN, false negatives; FNR, false negative rate.