Table 2.

Long primers designed to amplify the BAC/YAC pGF vector to add unique 3’ and 5’ hook sequences for the target DNA fragment in the HvAV-3h genome. The overlap size in base pairs (bp) between the medium-size fragments is provided

Target region	Primer name	Sequence 5’ > 3’	Overlap (bp)
Part 1	pGF-F–A8	TGAGCGGTCCCATAGGTTTTAGTTCTTCACACGGTAGCACGCCCGGGCgctagagtcgacctgcaggcatg	155
	pGF-R–A8	CCCGAAGAAACTTTCATTAAACTGTTCGTGGAGTCTTTACGCCCGGGCcgggtaccgagctcgaattc
Part 2	pGF-F–A9	TATGATCCGCGTATAACACCTCTGGATTGTGTTGTTCGTGGCCCGGGCgctagagtcgacctgcaggcatg	178
	pGF-R–A9	ACGTCGTTCGCTATGAGAGAGTGTTGCGAGAATTCCGATCGCCCGGGCcgggtaccgagctcgaattc
Part 3	pGF-F-A10	TTCGCCGGTAGCGAGCTGATGTTTGTAATGTATCATTTCGGCCCGGGCgctagagtcgacctgcaggcatg	–
	pGF-R-A10	CGCATAAAATCGTGTATAAAGCAGAACGGGAGAATAAGAGGCCCGGGCcgggtaccgagctcgaattc
pGF Vector	pGF-F*	TCGTCTCGCGCGTTTCGGTGATGACG	–
	pGF-R	GTCTGCTCCCGGCATCCGCTTACAGAC

*The pGF-F and pGF-R primers were used for the amplification of the pGF vector with the long primers to amplify the vector as two fragments