Fig. 3.
Nucleotide sequences of the TM2 region of the B. germanica Rdl gene, which includes the point mutation that results in the A302S substitution. (A) Representative sequences from the insecticide-susceptible Orlando Normal strain and five field-collected German cockroach populations were aligned against the reference sequence (MW267921.1), with the A302S region highlighted. (B) Representative direct sequencing chromatograms of homozygous susceptible (G/G) and homozygous resistant (T/T) cockroaches. The G-to-T point mutation site is shown within a box. MW267921.1 is the GenBank accession number for the B. germanica GABA-gated chloride channel complete cds (Jones et al. 2021).
