Key resources table.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| HRP conjugated anti human IgG antibody | Southern Biotech | Cat#204205 |
| anti human IgE antibody | Southern Biotech | Cat#9250-05 |
| mouse anti-human IgG Fc-BIOT | Southern Biotech | Cat#9040-08 |
| goat anti-human IgA-BIOT | Southern Biotech | Cat# 2050-08 |
| Bacterial and virus strains | ||
| T7Select 10-3 cloning kit | Merck | Cat#70550-3 |
| Biological samples | ||
| 1,054 serum samples of 1,010 individuals | Korem et al., 2017; Zeevi et al., 2015 | n.a. |
| Chemicals, peptides, and recombinant proteins | ||
| IPEGAL CA 630 | Sigma-Aldrich | Cat#I3021 |
| Protein A magnetic beads | Thermo Fisher Scientific | Cat#10008D |
| Protein G magnetic beads | Thermo Fisher Scientific | Cat#10009D |
| 1-Step™ Turbo TMB-ELISA Substrate Solution | Rhenium | Cat#TS-34022 |
| Q5 polymerase | New England Biolabs | Cat#M0493L |
| Bovine Serum Albumin, heat shock fraction, pH 7, ≥98% | Sigma-Aldrich / Merck | Cat#A7906-100G |
| Pierce Streptavidin Magnetic Beads | ThermoFisher | Cat#88817 |
| Critical commercial assays | ||
| QIAquick gel extraction kit | Qiagen | Cat#28704 |
| QIAquick PCR purification kit | Qiagen | Cat#28104 |
| Deposited data | ||
| Raw and analyzed data | This paper |
https://doi.org/10.6084/m9.figshare.17098958
https://doi.org/10.7910/DVN/3SOZCQ |
| Code | This paper. | DOI: 10.5281/zenodo.7307894 |
| Experimental models: Cell lines | ||
| Experimental models: Organisms/strains | ||
| Oligonucleotides | ||
| library amplification primer fwd | GATGCGCCGTGGGAATTCT | n.a. |
| library amplification primer rev | GTCGGGTGGCAAGCTTTCA | n.a. |
| Recombinant DNA | ||
| Oligo pool (200 mers) | Twist Bioscience | n.a. |
| Software and algorithms | ||
| See data availability statement. | ||
| Other | ||
| Nunc™ Immobilizer™ Streptavidin Plates | Thermo Scientific™ | Cat#436014 |
| BioTides™ Peptides | JPT Peptide Technologies (Berlin, Germany) | n.a. |
| Freedom Evo liquid handling robot with | Tecan | n.a. |
| FrameStar® 96, fully skirted, white frame, 50 plates | Tamar | Cat#4ti-0960/W |
| MASTERBLOCK, 96w, PP, 2ml, Natural, 50/case | Danyel biotech | Cat#60-780270 |
| Corning Axygen® AM-2ML-SQ AxyMat™ | Biolab Ltd | Cat#AXY-AM-2ML-SQ |