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. 2025 May 12;16:1541714. doi: 10.3389/fendo.2025.1541714

Table 5.

Literature studies on the regulation of osteoporosis by oxidative stress mediated by the PI3K/Akt/mTOR signalling pathway.

Intervention measures Induction model Animal/cell source Ethical review Signalling pathway Research results Literature source
Tocopherol H2O2 BMSCs Yes By inhibiting the phosphorylation levels of PI3K, Akt and mTOR Maintain cell viability and reduce ROS levels in H2O2-stimulated BMSCs Lan (77)
IL-15 Osteoclast Yes Increase the expression of p-Akt and mTOR Promoting the secretion of IL-15 can increase the level of oxidative stress and facilitate the proliferation and differentiation of osteoclasts Shi (78)
Leonurine H2O2 OVX Rat/BMSCs Yes Inhibit the activation of PI3K/Akt/mTOR Reduce H2O2-induced ROS production, and decrease the levels of intracellular reactive oxidation markers NOX4 and COX2 Zhao (79)
Monotropein H2O2 Rat Osteoblasts Yes By inhibiting the phosphorylation of Akt and the signalling of mTOR Attenuated the H2O2-induced oxidative stress Shi (80)
TFRD+ CaCO3 OVX Rat Yes Reduced the levels of phosphorylated proteins of PI3K/Akt/mTOR in the oxidative stress pathway of rats in the osteoporosis model Reduced the levels of oxidative stress-related markers in the serum Mu (81)
Quercetin H2O2 SD Rat/BMSCs Yes After quercetin treatment, the phosphorylation levels of PI3K, Akt and mTOR were downregulated Downregulate the ROS level and upregulate the expression of antioxidant genes Lan (82)
PQQ+E2 OVX C57BL/6 mouse Yes OVX mice the ROS level and MDA content decreased significantly, while the T-AOC, SOD activity, and the protein expression levels of SOD1, SOD2, and Sirt1 increased significantly Geng (83)
Curcumin Rat Yes Activate the PI3K/Akt signalling pathway Promote the production of ROS and inhibit the expression of FGF23, thereby promoting bone anabolism and the regeneration process Marcucci (84)

E2, Estradiol; MDA, Malondialdehyde; T-AOC, Total Antioxidant Capacity; SOD, Superoxide Dismutase; TFRD, Total flavonoids of Rhizoma Drynariae; PQQ, Pyrroloquinoline quinone.