Fig. 1.

Generation of 3D8‐CDR L1‐grafted Abs. (A) Schematic representation of D1 Ab generation by replacing the CDR L1 region with the 3D8‐CDR L1 sequence. (B) Amino acid sequences of the light chain FR1‐CDR1‐FR2 region, aligned according to the Kabat and IMGT numbering systems. The 3D8 CDR1 sequence is highlighted in yellow, while the grafted CDR L1 sequences in D1 Abs are shown in gray. (C) SDS/PAGE analysis of purified parental and D1 Abs (2 μg) under reducing and non‐reducing conditions using a 4–20% gradient polyacrylamide gel, with proteins visualized by Coomassie brilliant blue staining.