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. 2025 May 11;14(10):692. doi: 10.3390/cells14100692
CHO Chinese Hamster Ovary (cells)
CRISPR/Cas9 Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated protein 9
DHFR Dihydrofolate Reductase (selection marker gene)
GS Glutamine Synthetase (selection marker gene)
Bak1 BCL2 Antagonist/Killer 1 (pro-apoptotic gene)
Bax BCL2-Associated X Protein (pro-apoptotic gene)
Bcl-2 B-cell Lymphoma 2 (anti-apoptotic gene)
Beclin-1 Autophagy-related protein (enhances macroautophagy)
NGS Next-Generation Sequencing (high-throughput DNA sequencing)
gRNA Guide RNA (used in CRISPR/Cas9 for targeting specific DNA sequences)
MTX Methotrexate (DHFR inhibitor used for gene amplification)
ELISA Enzyme-Linked Immunosorbent Assay (protein detection method)
RT-PCR Reverse Transcription Polymerase Chain Reaction (gene expression analysis)
qPCR Quantitative Polymerase Chain Reaction (quantitative gene expression analysis)
Fc Fragment crystallizable (region of an antibody)
IgG Immunoglobulin G (antibody type)
mAb Monoclonal Antibody
EEF1A1 Eukaryotic Translation Elongation Factor 1 Alpha 1 (promoter for gene expression)
PAM Protospacer Adjacent Motif (CRISPR/Cas9 target sequence requirement)
ORF Open Reading Frame (coding sequence of a gene)
SNP Single Nucleotide Polymorphism (genetic variation)
Indel Insertion/Deletion (genetic mutation)
CDS Coding DNA Sequence (region of DNA that codes for a protein)
LC3 Microtubule-associated protein light chain 3 (autophagy marker)
Apaf-1 Apoptotic Protease Activating Factor 1 (apoptosis-related protein)
XIAP X-linked Inhibitor of Apoptosis Protein (apoptosis inhibitor)
ULK1 Unc-51 Like Autophagy Activating Kinase 1 (autophagy-related kinase)
ATG Autophagy-related Gene (genes involved in autophagy)
VPS34 Vacuolar Protein Sorting 34 (autophagy-related kinase)
AMPK AMP-activated Protein Kinase (energy-sensing enzyme)
mTOR Mechanistic Target of Rapamycin (regulates cell growth and autophagy)
FIP200 Focal Adhesion Kinase Family Interacting Protein of 200 kD (autophagy-related protein)
Ambra1 Autophagy and Beclin-1 Regulator 1 (autophagy-related protein)
PE Phosphatidylethanolamine (lipid involved in autophagy)
PAR Poly(ADP-ribose) (marker of parthanatos, a form of cell death)
FSP1 Ferroptosis Suppressor Protein 1 (marker of ferroptosis, a form of cell death)
MDA Malondialdehyde (marker of lipid peroxidation in ferroptosis)
GPX4 Glutathione Peroxidase 4 (enzyme that prevents ferroptosis)
ER Endoplasmic Reticulum